Isolated nucleic acid molecules associated with gastric cancer and methods for diagnosing and treating gastric cancer

ABSTRACT

Various molecules associated with disorders such as gastric cancer are disclosed. The invention also discloses diagnostic and therapeutic methods based upon these molecules.

RELATED APPLICATIONS

[0001] This application is a divisional of application Ser. No. 08/896,164, filed Jul. 17, 1997, now pending. This application claims the benefit under 35 U.S.C. §120 of application Ser. No. 08/896,164.

FIELD OF THE INVENTION

[0002] This invention relates to the isolation of genes associated with gastric cancer, methods of diagnosing gastric cancer using these, as well as other genes which are known, as well as therapeutic approaches to treating such conditions.

BACKGROUND OF THE INVENTION

[0003] It is fairly well established that many pathological conditions, such as infections, cancer, autoimmune disorders, etc., are characterized by the inappropriate expression of certain molecules. These molecules thus serve as “markers” for a particular pathological or abnormal condition. Apart from their use as diagnostic “targets”, i.e., materials to be identified to diagnose these abnormal conditions, the molecules serve as reagents which can be used to generate diagnostic and/or therapeutic agents. A by no means limiting example of this is the use of cancer markers to produce antibodies specific to a particular marker. Yet another non-limiting example is the use of a peptide which complexes with an MHC molecule, to generate cytolytic T cells against abnormal cells.

[0004] Preparation of such materials, of course, presupposes a source of the reagents used to generate these. Purification from cells is one laborious, far from sure method of doing so. Another preferred method is the isolation of nucleic acid molecules which encode a particular marker, followed by the use of the isolated encoding molecule to express the desired molecule.

[0005] To date, two strategies have been employed for the detection of such antigens, in e.g., human tumors. These will be referred to as the genetic approach and the biochemical approach. The genetic approach is exemplified by, e.g., de Plaen et al., Proc. Natl. Acad. Sci. USA 85: 2275 (1988), incorporated by reference. In this approach, several hundred pools of plasmids of a cDNA library obtained from a tumor are transfected into recipient cells, such as COS cells, or into antigen-negative variants of tumor cell lines. Transfectants are screened for the expression of tumor antigens via their ability to provoke reactions by anti-tumor cytolytic T cell clones. The biochemical approach, exemplified by, e.g., Mandelboim, et al., Nature 369: 69 (1994) incorporated by reference, is based on acidic elution of peptides which have bound to MHC-class I molecules of tumor cells, followed by reversed-phase high performance liquid chromography (HPLC). Antigenic peptides are identified after they bind to empty MHC-class I molecules of mutant cell lines, defective in antigen processing, and induce specific reactions with cytolytic T-lymphocytes (“CTLs”). These reactions include induction of CTL proliferation, TNF release, and lysis of target cells, measurable in an MTT assay, or a ⁵¹Cr release assay.

[0006] These two approaches to the molecular definition of antigens have the following disadvantages: first, they are enormously cumbersome, time-consuming and expensive; second, they depend on the establishment of CTLs with predefined specificity; and third, their relevance in vivo for the course of the pathology of disease in question has not been proven, as the respective CTLs can be obtained not only from patients with the respective disease, but also from healthy individuals, depending on their T cell repertoire.

[0007] The problems inherent to the two known approaches for the identification and molecular definition of antigens is best demonstrated by the fact that both methods have, so far, succeeded in defining only very few new antigens in human tumors. See, e.g., van der Bruggen et al., Science 254: 1643-1647 (1991); Brichard et al., J. Exp. Med. 178: 489-495 (1993); Coulie, et al., J. Exp. Med. 180:35-42 (1994); Kawakami, et al., Proc. Natl. Acad. Sci. USA 91:3515-3519 (1994).

[0008] Further, the methodologies described rely on the availability of established, permanent cell lines of the cancer type under consideration. It is very difficult to establish cell lines from certain cancer types, as is shown by, e.g., Oettgen, et al., Immunol. Allerg. Clin. North. Am. 10:607-637 (1990). It is also known that some epithelial cell type cancers are poorly susceptible to CTLs in vitro, precluding routine analysis. These problems have stimulated the art to develop additional methodologies for identifying cancer associated antigens.

[0009] One key methodology is described by Sahin, et al., Proc. Natl. Acad. Sci. USA 92: 11810-11913 (1995), incorporated by reference. Also, see U.S. patent applications Ser. No. 08/580,980, and application Ser. No. 08/479,328, filed on Jun. 7, 1995 and Jan. 3, 1996, respectively. All three of these references are incorporated by reference. To summarize, the method involves the expression of cDNA libraries in a prokaryotic host. (The libraries are secured from a tumor sample). The expressed libraries are then immunoscreened with absorbed and diluted sera, in order to detect those antigens which elicit high titer humoral responses. This methodology is known as the SEREX method (“Serological identification of antigens by Recombinant Expression Cloning”). The methodology has been employed to confirm expression of previously identified tumor associated antigens, as well as to detect new ones. See the above referenced patent applications and Sahin, et al., supra, as well as Crew, et al., EMBO J 144:2333-2340 (1995).

[0010] The SEREX methodology has now been applied to stomach cancer samples. Several nucleic acid molecules have been newly isolated and sequenced, and are now associated with stomach cancer. Further, a pattern of expression involving these, as well as previously isolated genes has been found to be associated with stomach cancer. These results are the subject of this application, which is elaborated upon in the disclosure which follows.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS EXAMPLE 1

[0011] A stomach cancer cDNA library was established, using standard techniques, then the library was screened, using the SEREX methodology described supra, and set forth by Sahin et al., Proc. Natl. Acad. Sci. USA 92:11810 (1995), and by Chen et al., Proc. Natl. Acad. Sci. USA 94:1914 (1997), incorporated by reference in their entirety.

[0012] To be specific, total RNA was isolated by homogenizing tumor samples in 4 M guanidium thiocyanate/0.5% sodium N-lauryl sarcosine/ and 25 mM EDTA followed by centrifugation in 5.7 M CsCl/25 mM sodium acetate/10 uM EDTA at 320,000 rpm. Total mRNA was removed by passing the sample over an oligo-dT cellulose column. The cDNA libraries were then constructed by taking 5 μg of mRNA, using standard methodologies to reverse transcribe the material.

[0013] Libraries were prepared from three different patients, referred to as “SM”, “CK” and “SS” respectively. A total of 2.5×10⁶, 1.1×10⁶, and 1.7×10⁶ cDNA clones were obtained from the individuals.

[0014] The cDNA was used to construct a lambda phage library, and 500 phages were plated onto XL1-Blue MRF E. coli, and incubated for eight hours at 37° C. A nitrocellulose membrane was then placed on the plate, followed by overnight incubation. The membrane was then washed, four times, without TBS which contained 0.05% Tween, and was then immersed in TBS containing 5% non-fat dried milk. After one hour, the membrane was incubated with conjugates of peroxidase-goat anti human IgG specific for Fc portions of human antibody (1:2000, diluted in TBS with 1% BSA. The incubation was carried out for one hour, at room temperature, and the membrane was then washed three times with TBS. Those clones which produced antibodies were visualized with 0.06%, 3,3′diamino benzidine tetrachloride, and 0.015% H₂O₂, in 50 mM Tris (pH 7.5). Any clones which produced immunoglobulin were marked, and then the membrane was washed, two further times, with TBS that contained 0.05% Tween, and then twice with “neat” TBS.

[0015] The membranes were then incubated in 1:100 diluted patient serum, overnight, at 4° C. The patient serum had been pretreated. Specifically, 5 ml samples were diluted to 10 ml with TBS containing 1% bovine serum albumin, and 0.02% Na₃N. The serum had been treated to remove antibodies to bacteriophage, by passing it through a 5 ml Sepharose column, to which a lysate of E. coli Y1090 had been attached, followed by passage over a second column which had E. coli lysate and lysate of E. coli infected with lambda bacteriophage. The screening was carried out five times. The samples were then diluted to 50 ml, and kept at −80° C., until used as described herein.

[0016] Following the overnight incubation with the membrane, the membrane was washed twice with TBS/0.05% Tween 20, and then once with TBS. A further incubation was carried out, using the protocols discussed supra, for the POD labelled antibodies.

[0017] In the case of library SM, 55 positive clones were obtained. Library CK yielded 56 positive clones after the first screening, and after two additional screenings, this number was reduced to 26.

[0018] The 55 positive clones were then sequenced, using standard techniques. Following comparison of the sequences to information available in data banks, a total of 36 clones were resolved into known and unknown genes. In the table that follows, the “+” and “−” signs are essentially used to compare signals to each other. All were positive. Table 1, which follows, summarizes some of this work. Specifically, with reference to the first page of the table, previously identified human proteins and the nucleotide sequences, set forth in SEQ ID NOS: 1 to 39 are known. The four molecules which follow (gelsolin, zinc finger protein family, variant zinc finger motif protein goliath and homeodomain proteins, have not been identified in humans previously, although there are related molecules found in other species. SEQ ID NOS: 40 and 48 set forth these nucleotides sequences. Finally, with reference to the table the last four moieties, i.e., prepro-α collagen, heterogeneous ribonucleoprotein D, nucleosome assembly protein 2, and NY-ESO-2/Ulsn NRP/V1 small nuclear ribonucleoprotein, are also known. Nucleotide sequences are set forth at SEQ ID NOS: 49 to 55. The nucleic acid molecules having the nucleotide sequences set forth at SEQ ID NOS: 56 to 83 represent molecules for which no related sequences were found. SEQ ID NO: 84 combines the sequences of SEQ ID NOS: 40 to 43, inclusive. SEQ ID NO: 85 combines SEQ ID NOS: 56-69, SEQ ID NO: 86 combines SEQ ID NOS: 70, 72 and 75, while SEQ ID NO: 87 combines SEQ ID NOS: 71, 73, 74 and 76. TABLE 1 Insert Clone SEQ ID NO Reactivity size RPB-JK S366 1, 2 ++ 1.75 Human H-2K S224 3 ++ 1.56 Binding Factor 2 S541 4 ++ 1.98 (7 clones) S584 5 ++ 1.86 S194 6 ++-+ 2.43 S344 7, 8 + 1.69 S699 9 + 1.85 Telomeric Repeat Binding S385 10, 11 ++ 0.96 Protein (2 clones) S4510 12 ++ 0.96 Protein Kinase B/AKT/ S621 13 ++ 2.00 Serine Threonine Protein S641 14 + 1.80 Kinase rac alpha (2 clones) SRY Interacting Protein-1/ S72 15, 16 ++ 1.70 Tyrosine Kinase Activator S321 17, 18 ++ 1.76 Protein (2 clones) Sterol Carrier Protein-X/ S571 19 ++ 2.20 Sterol Carrier Protein-2 S613 20 ++ 2.25 (3 clones) S62 21, 22 + 2.91 Archain/Coat Protein S537 23, 24 ++ 3.42 Delta-Cop (Bovine) S632 25 ++ 0.90 (3 clones) S591 26 ++-+ 3.50 HEM-1 S551 27, 28 ++ 1.95 (2 clones) S634 29 ++ 3.46 Id-1 Helix-Loop-Helix S271 30, 31 +++ 1.21 Protein (1 clone) E2A Helix-Loop-Helix S231 32, 33 ++ 1.66 Transcription Factor (1 clone) Follistatin-Related Protein S706 34 + 3.60 (1 clone) Translation Initiation S274 35, 36 + 3.82 Factor eIF-4γ (1 clone) M Phase Phosphoprotein 1 S204 37, 38 + 4.71 (1 clone) Lysyl tRNA Synthetase S691 39 + 1.80 (1 clone) Gelsolin Family S564 40, 41 ++ 2.04 (2 clones) S26 42, 43 + 2.57 Zinc Finger S343 44, 45 ++-+ 0.90 Protein Family (1 clone) A Variant Zinc-Finger- S622 46 ++-+ 1.70 Motif Protein Goliath Homeodomain Protein S4611 47, 48 + 3.20 Family (1 clone) Prepro-α1 (1) S563 49 ++ 3.28 Collagen (1 clone) Heterogeneous S292 50 + 1.25 Ribonucleoprotein D S222 51 + 2.49 (3 clones) S232 52 + 1.60 Nucleosome Assembly S524 53, 54 + 1.52 Protein 2 (1 clone) NY-ESO-2/U1sn NRP S623 55 + 0.9 U1 Small Nuclear Ribonucleoprotein (1 clone)

[0019] The foregoing examples demonstrate several features of the invention. These include diagnostic methods for determining presence of transformed cells, such as gastric cancer cells, in a sample. The sample may contain whole cells or it may be, e.g., a body fluid sample, or an effusion, etc., where the sample may contain cells, but generally will contain shed antigen. The experiments indicate that there is a family of proteins, expression of which is associated with gastric cancer. Hence, the invention involves, inter alia, detecting at least two of the proteins set out in Table 1 wherein, presence of these is indicative of a pathology, such as gastric cancer or other type of related condition. Exemplary of the type of diagnostic assays which can be carried out are immunoassays, amplification assays (e.g., PCR), or, what will be referred to herein as a “display array”. “Display array” as used herein refers to a depiction of the protein profile of a given sample. Exemplary of such displays are 2-dimensional electrophoresis, banding patterns such as SDS-gels, and so forth. Thus, one aspect of the invention involves diagnosing gastric cancer or a related condition by determining protein display of a sample, wherein a determination of at least two of the proteins, or expression of their genes, as set forth in Table 1, is indicative of gastric cancer or a related condition. There are many ways to carry out these assays. For example, as indicated herein, antibodies to the proteins were found in patient samples. One can assay for these antibodies using, e.g., the methodology described herein, or by using a purified protein or proteins or antigenic fragment thereof, and so forth. One can also assay for the protein itself, using antibodies, which may be isolated from samples, or generated using the protein and standard techniques. This antibodies can then be labelled, if desired, and used in standard immunoassays. These antibodies or oligonucleotide probes/primers may also be used to examine biopsied tissue samples, e.g., to diagnose precancerous conditions, early stage cancers, and so forth.

[0020] Similarly, any and all nucleic acid hybridization systems can be used, including amplification assays, such as PCR, basic probe hybridization assays, and so forth. The antibodies, such as polyclonal antibodies, monoclonal antibodies, the hybridomas which produce them, recombinantly produced antibodies, binding fragments of these, hybridization kits, DNA probes, and so forth, are all additional features of the invention.

[0021] Any of these assays can also be used in progression/regression studies. One can monitor the course of an abnormality such as gastric cancer which involve expression of any one of the proteins, the expression of which is governed by nucleic acid molecules which comprise SEQ ID NOS: 40-48 and 56-87, simply by monitoring levels of the protein, its expression, and so forth using any or all of the methods set forth supra.

[0022] As has been indicated supra, the isolated nucleic acid molecules which comprise the nucleotide sequences set forth in SEQ ID NOS: 40-48 and 56-87 are new, in that they have never been isolated before. These nucleic acid molecules may be used as a source to generate gastric cancer specific proteins and peptides derived therefrom, and oligonucleotide probes which can themselves be used to detect expression of these genes. Hence, a further aspect of the invention is an isolated nucleic acid molecule which comprises any of the nucleotide sequences set forth in SEQ ID NOS: 40-48 and 56-87, or molecules whose complements hybridize to one or more of these nucleotide sequences, under stringent conditions, expression vectors comprising these molecules, operatively linked to promoters, cell lines and strains transformed or transfected with these, and so forth. “Stringent conditions”, is used herein, refers to condition such as those specified in U.S. Pat. No. 5,342,774, i.e., 18 hours of hybridization at 65° C., followed by four one hour washes at 2xSSC, 0.1% SDS, and a final wash at 0.2xSSC, more preferably 0.1xSSC, 0.1% SDS for 30 minutes, as well as alternate conditions which afford the same level of stringency, and more stringent conditions.

[0023] It should be clear that these methodologies may also be used to track the efficacy of a therapeutic regime. Essentially, one can take a baseline value for the protein or proteins being tested, using any of the assays discussed supra, administer a given therapeutic, and then monitor levels of the protein or proteins thereafter, observing changes in protein levels as indicia of the efficacy of the regime.

[0024] For those proteins which have been identified previously, i.e., those in Table 1, similar assays can be carried out, but for two or more of the proteins or nucleic acid molecules encoding them. Specifically, tilomeric repeat binding protein, or protein kinase B/AKT/serine threonine protein kinase rac alpha are preferably determined, either in the form of the protein per se, or nucleic acid molecules encoding them (e.g., SEQ ID NOS: 10-14), together, or in combination with one or more of human gelsolin (SEQ ID NOS: 40-43 and 84), one or more of SEQ ID NOS: 56-76, 85, 86 or 87), or the proteins encoded by these sequences, and so forth.

[0025] The identification of the proteins set forth herein as being implicated in pathological conditions such as gastric cancer also suggests a number of therapeutic approaches to such conditions. The experiments set forth supra establish that antibodies are produced in response to expression of these proteins, suggesting their use as a vaccine. Hence, a further embodiment of the invention is the treatment of conditions which are characterized by expression of one or more of the subject proteins, via immunotherapeutic approaches. One of these approaches is the administration of an amount of one or more these proteins, or an immunogenic peptide derived therefrom in an amount sufficient to provoke or augment an immune response. The proteins or peptides may be combined with one or more of the known immune adjuvants, such as saponins, GM-CSF, interleukins, and so forth. If the peptides are too small to generate a sufficient antibody response, they can be coupled to the well known conjugates used to stimulate responses.

[0026] Similarly, the immunotherapeutic approaches include administering an amount of inhibiting antibodies sufficient to inhibit the protein or proteins. These antibodies may be, e.g., antibodies produced via any of the standard approaches elaborated upon supra.

[0027] T cell responses may also be elicited by using peptides derived from the proteins which then complex, non-covalently, with MHC molecules, thereby stimulating proliferation of cytolytic T cells against any such complexes in the subject. It is to be noted that the T cells may also be elicited in vitro, and then reperfused into the subject being treated.

[0028] Note that the generation of T cells and/or antibodies can also be accomplished by administering cells, preferably treated to be rendered non-proliferative, which present relevant T cell or B cell epitopes for response.

[0029] The therapeutic approaches may also include gene therapies, wherein an antisense molecule, preferably from 10 to 100 nucleotides in length, is administered to the subject either “neat” or in a carrier, such as a liposome, to facilitate incorporation into a cell, followed by inhibition of expression of the protein. Such antisense sequences may also be incorporated into appropriate vaccines, such as in viral vectors (e.g., Vaccinia), bacterial constructs, such as variants of the well known BCG vaccine, and so forth.

[0030] An additional DNA based therapeutic approach is the use of a vector which comprises one or more nucleotide sequences, preferably a plurality of these, each of which encodes an immunoreactive peptide derived from the expressed proteins. One can combine these peptides expressing sequences in all possible variations, such as one from each protein, several from one or more protein and one from each of the additional proteins, a plurality from some and none from others, and so forth.

[0031] Other features of the invention will be clear to the skilled artisan, and need not be repeated here.

[0032] The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, it being recognized that various modifications are possible within the scope of the invention.

1 87 1 707 DNA Homo sapiens Unsure (107)..(107) n = a, c, g or t 1 agatggcgcc tgttgtgaca gggaaatttg gtgagcggcc tccacctaaa cgacttacta 60 gggaagctat gcgaaattat ttaaaagagc gaggggatca aacagtnctt attcttcatg 120 caaaagttgc acagaagtca tatggaaatg aaanaaggtt tttttgccca cctccttgtg 180 tatatcttat gggcantgga tggaagaaaa aaaangaaca aatggaacgc gatggttgtt 240 ctgaacaaaa gtctcaaccg tgtgcattta ttgggatagg aaatagtgac caaaaaatgc 300 agcagctana cttggaagga aagaactatt gcacagccaa aacattgtat atatctgact 360 cagacaagcg aaagcacttc atgttgtctg taaagatgtt ctatggcaac agtgatgaca 420 ttggtgtgtt cctcagcaag cgaataaaag tcntctccaa accttccaaa aagaacagtc 480 attgaaaaat gctgacttat gcattgcctc angaacaaag gtggctctgt ttaatcgact 540 acnatcccan acagttagta ccagatactt gcatgttana aggaggtnat tttcatgcca 600 gttcacagcn gtggggagcc ttttttattc anctcttgga tgatgatgan tccnaaggag 660 aagaattcac ngtccgagat ggctacatcc attatggaca aacagtc 707 2 551 DNA Homo sapiens Unsure (200)..(200) n = a, c, g or t 2 actgtggctt ctgcatttca aatcagcact tgcagggaga caacggggtt tttgaatagt 60 atcacctggt atgaaaagtt ttcccaagaa accacaaacg attgttcatt ttttctcctt 120 ttttgttaac tttttgccac actcaagtca gtttaagtcc tagcaaaaag acggtagtta 180 ggataccact gtggctgtan atgatgtgac actggttgaa tttgtgctgg cgtttgtgta 240 acttccctcg ctgtttgtgt ttgattcgtt agggggcacc tggcttgaat tggctcgaag 300 gattgctcct gctgcactgc aatgtggccg cggccctggt tctggtgtgt angtaaaggt 360 aaggctggtg gaataaatga ttccaccatt tcggaccaaa gttactggaa cctggactgg 420 ttgccggacc catctccaac cttctcggaa tgcanaaatg tctgggacga cacagaacat 480 acctctccac acctgtacat aatttcagct tctacatccc caaaccacac tcgtaaattt 540 ggantnaaaa t 551 3 478 DNA Homo sapiens Unsure (39)..(39) n = a, c, g or t 3 actgtggctt ctgcatttca aatcagcact tgcagggana caacggggtt tttgaatagt 60 atcacctggt atgaaaagtt ttcccaagaa accacaaacn attgttcatt ttttctcctt 120 ttttgttaac ttttngccac actcaantca gtttaagtcc tagcaaaaan acggtagtta 180 ggataccact gtggctgtaa atgatntgac actggttgaa tttgtgctgg cgtttgtgta 240 acttccctcg ctgtttgtgt ttgattcgtn agggggcacc tggcttgaat tggctcgaag 300 gattgctcct gctgcactgc aatgtggccg cgggcctgnt tcttatntgt tgtaaangtn 360 aggntggtgg aataaatgat tccatcatnt cggancgaag ttgctgggaa ctggganngg 420 tngncggaac catctccgac cncccggaaa ngcagaagtg ttngtggnag accggaac 478 4 707 DNA Homo sapiens Unsure (88)..(88) n = a, c, g or t 4 actgtggctt ctgcatttca aatcagcact tgcagggaga caacggggtt tttgaatagt 60 atcacctggt atgaaaagtt ttcccaanaa accacaaacg attgttcatt ttttctcctt 120 ttttgttaac tttttgccac actcaantca gtttaantcc tancaaaaag acggtagtta 180 ggataccact gtggctgtaa atgatgtgac actggttgaa tttgtgctgg cgtttgtgta 240 acttccctcg ctgtttgtgt ttgattcgtt agggggcacc tggcttgaat tggctcgaag 300 gattgctcct gctgcactgc aatgtggccg cggccctggt tctggtgtgt aggtaaaggt 360 aaggctggtg gaataaatga ttccatcatt tcggaccaaa gttactggaa cctggactgg 420 ttgccggacc catctccaac cttctcggaa tgcagaaatg tctgggacga cacagancat 480 actctctcca cacctgtaca tagtttcngc ttctacatcc ccaaaccaca ctcgtaaatt 540 tggantgaaa ttctgtcctg taagttcaag cattnctacg tccccacccg ccatttcaac 600 tgaaaggctc tctaccacan ggnacaggaa atgactgggg caaggacagg gcccattccc 660 tcattaaatg tnatactccg ccttatcngt cctaaangaa tgtncaa 707 5 541 DNA Homo sapiens Unsure (18)..(18) n = a, c, g or t 5 ggtaaacttt tggccacncn caattcantt taattcctac caaaaaaacg gtatttagna 60 tnccnctgtg gctgtaaata atttaacnct ggttaaattn ntnctggctt tngtntanct 120 cccccccctn ttngtttttn atccnttagg gggcacctgn cttnantngg cncaaaggat 180 ngcccctgct gcantgcaat ttggccncgg ccctggtcct ggtttntagg taaaggtaag 240 gcnggtgnaa taantaatcc caccattncg naccaaattt actgnaacct gaacnggttg 300 ccgnacccan cnccancctn cncgaaatgc aaaantttct ggnacaacnc aaaccntacn 360 cncnccaccc ctntncntat ttncagctnc tacntcccca aaccacacnc ntaaattngn 420 attaaaatcc tntcctgtaa ttccaagcat ggctacttcc ccaccgccat tcaactnaag 480 gcccnctacc acaggcncag nattaantgg ggcaaggaaa gggcccatcc ccccataaaa 540 t 541 6 605 DNA Homo sapiens Unsure (39)..(39) n = a, c, g or t 6 actgtggctt ctgcatttca aatcagcact tgcagggana caacggggtt tttgaatant 60 atcacctggt atgaaaagtt ttcccaanaa accacaaacn antgttcatt tttnctcctt 120 ttttgttaac tttttgccac actcaantca gtttaantcc tagcaaaaaa acggtagtta 180 ggataccact gtggctgtaa atgatgtnac actggttgaa tttgtgctgg cgtttgtgtn 240 acttccctcg ctgtttgtgt ttgattcgtt agggggcacc tggcttgaat tggctcgaan 300 gattgctcct gctgcactgc aatgtggccg cggccctggt tctggtgtgt aagtaaaggt 360 aaggctggtg gaataaatga ttccntcatt tcggancaaa gttactggaa cctggantgg 420 ttgncggacc atctccaacc ttctcggaat gcanaaatgt ctgggacaan acnnaacata 480 ctctctccnc acctggttca tantttcagc ttctacatcc cccaaaccac actcntaaat 540 ttggantgaa attctgtcct gttaattcaa acattgctac gtccccnccg ccattcaact 600 gaaag 605 7 666 DNA Homo sapiens Unsure (450)..(450) n = a, c, g or t 7 gaagagtttg tggaagatgg cgcctgttgt gacagggaaa tttggtgagc ggcctccacc 60 taaacgactt actagggaag ctatgcgaaa ttatttaaaa gagcgagggg atcaaacagt 120 acttattctt catgcaaaag ttgcacagaa gtcatatgga aatgaaaaaa ggtttttttg 180 cccacctcct tgtgtatatc ttatgggcag tggatggaag aaaaaaaaag aacaaatgga 240 acgcgatggt tgttctgaac aagagtctca accgtgtgca tttattggga taggaaatag 300 tgaccaagaa atgcagcagc taaacttgga aggaaagaac tattgcacag ccaaaacatt 360 gtatatatct gactcagaca agcgaaagca cttcatgttg tctgtaaaga tgttctatgg 420 caacagtgat gacattggtg tgttcctcan caagcggata aaagtcatct ccaaaccttc 480 caaaaagaac agtcattgaa aaatgctgac ttatgcattg cctcaggaac aaaggtggct 540 ctgtttaatc gactacgatc ccagacagtt ngtaccagat acttgcatgt anaaggaggt 600 aattttccat gccagttccc accagtgggg agcctttttt attcnctctt gggatgatga 660 tgaatc 666 8 600 DNA Homo sapiens Unsure (65)..(65) n = a, c, g or t 8 gccacactca agtcagttta agtcctagca aaaagacggt agttaggata ccactgtggc 60 tgtanatgat gtgacactgg ttgaatttgt gctggcgttt gtgtaacttc cctcgctgtt 120 tgtgtttgat tcgttagggg gcacctggct tgaattggct cgaaggattg ctcctgctgc 180 actgcaatgt ggccgcggcc ctggttctgg tgtgtaggta aaggtaaggc tggtggaata 240 aatgattcca tcatttcgga ccaaagttac tggaacctgg actggttgcc ggacccatct 300 ccaaccttct cggaatgcag aaatgtctgg gacgacacag ancatactct ctccacacct 360 gtacatagtt tcagcttcta catccccaaa ccacactcgt aaatttggag tgaaattctg 420 tcctgtaagt tcaagcattg ctacgtcccc accgccattc aactgaaggc tctctaccac 480 aggcacagga atgactgggg caaggacagg gcccattccc tncataaaat gtntaatttg 540 gggncaantg tggcccccaa ccccccccca aagggcatna tttaacnccn ctttaattgg 600 9 835 DNA Homo sapiens Unsure (88)..(88) n = a, c, g or t 9 actgtggctt ctgcatttca aatcagcact tgcagggaga caacggggtt tttgaatagt 60 atcacctggt atgaaaagtt ttcccaanaa accacaaacn attgttcatt ttttctcctt 120 ttttgttaac tttttgccac actcaantca gtttaagtcc tagcaaaaan acggtagtta 180 ggataccact gtggctgtaa atnatgtgac actggttgaa tttgtgctgg cgtttgtgta 240 acttccctcg ctgtttgtgt ttgattcgtt agggggcacc tggcttgaat tggctcgaag 300 gattgctcct gctgcactgc aatgtggccg cggccctggt tctggtgtgt aggtaaaggt 360 aaggctggtg gaataaatga ttccatcatt tcggaccaaa gttactggaa cctggactgg 420 ttgccggacc catctccaac cttctcggaa tgcagaaatg tctgggacga cacanancat 480 actctctcca cacctgtaca tagtttcagc ttctacatcc ccaaaccaca ctcgtaaatt 540 tggagtgaaa ttctgtcctg taagttcaag cattgctacg tccccaccgc cattcaactg 600 aaggcctcta cacaggcaca ggaatgactg gggcaaggan agggcccatt ccctcataaa 660 atgtatactc tgccttatct gtgctaatga ttgtccagga aacgccanca ttttaccacc 720 tcnttattgg ttcttttggg antggaatgg cctgaaattg aaatattctt ccttgaaaaa 780 aggccaaata cntcttctgt ttccttnaag ggtaaaatgc ccatttttgg aattg 835 10 443 DNA Homo sapiens Unsure (105)..(105) n = a, c, g or t 10 agcagttcga atgccaggaa actgctcgag tgccaggtgc aggtgggggc ccccgaggag 60 gaggaggagg aggaggagga cgcgggcctg gtggccgagg ccgangccgt ggctgccggc 120 tggatgctcg atttcctctg cctctctctt tgccgagctt tccgcnacgg ccgctccgag 180 gacttccncn ggacccgcaa cagcgcanag gctattattc atggactatc cagtctaaca 240 gcttgccagt gagaacgata tacatatgtc agtttttgac aagaattgca gcaggaaaaa 300 cccttgatgc ncagtttgaa aatgatgaac gaattacacc cttggaatcn gccctgatga 360 tttggggttc aattgaaaag gaacatgacn aacttcntga agaaatacag aatttaatta 420 aaattcangc tatngctgtt tgt 443 11 491 DNA Homo sapiens Unsure (78)..(78) n = a, c, g or t 11 gtactttgag gagttcctac tcttctttct ttcttattaa ggtcttgttg ctgggttcca 60 tgttgcaact tagataanaa aagattcttg tgagacctca ataaggatac tgtaccctct 120 gaggattcag ttaccgcaga ctgtttgtca ctaacacttt ttcttgtatc caaattagct 180 tcagtttcca tttcaacatc attaccacta ggtttatctt gagaagttat tgttcttgtc 240 cttttgcttt ctactacttt tgccgctgcc ttcattagaa aggttgatga tttttcactt 300 agcacataat tcacataact cttaattttc tccatcatgt ggttgtagct gaagtgttga 360 aaaaaggaat gaaatgtatc tttctgagan attatcataa gcaatttgct tttgaaaggc 420 atatgagaat ttggatcacc aaatattctt tcaaagactt cttctgcttc tttaaagttg 480 ccattttcca t 491 12 802 DNA Homo sapiens Unsure (594)..(594) n = a, c, g or t 12 gtactttgag gagttcctac tcttctttct ttcttattaa ggtcttgttg ctgggttcca 60 tgttgcaact taaataagaa aagattcttg tgagacctca ataaggatac tgtaccctct 120 gaggattcag ttaccgcaga ctgtttgtca ctaacacttt ttcttgtatc caaattagct 180 tcagtttcca tttcaacatc attaccacta ggtttatctt gagaagttat tgttcttgtc 240 cttttgcttt ctactacttt tgccgctgcc ttcattagaa aggttgatga tttttcactt 300 agcacataat tcacataact cttaattttc tccatcatgt ggttgtagct gaagtgttga 360 aaaaaggaat gaaatgtatc tttctgagag attatcataa gcaatttgct tttgaaaggc 420 atatgagaat ttggatcacc aaatattctt tcaaagactt cttctgcttc tttaaagttg 480 ccattttcca tacaaacagc tatagcctga attttaatta aattctgtat ttcttcatga 540 agtttgtcat gttccttttc aattgaaccc caaatcatca gggctgattc caanggtgta 600 attcgttcat cattttcaaa ctgtgcatca agggtttttc ctgctgcaat tcttgtcaaa 660 aactgacata tgtntatcgt tctcaactgg cnagcctgtt aaactggaaa atccatgaat 720 aataacctct ggcgctgttg cgggtcctgc ggaaattccn cggaaccggc cgtcncggaa 780 aactcngcaa aagaaaaaaa gc 802 13 523 DNA Homo sapiens Unsure (21)..(21) n = a, c, g or t 13 gaaaagcaac ttttattgaa naatttggag ggaaggttcc atattatatt ataatagtaa 60 aaatactaaa gttgaatgtt gtaaaaaaac nccgtggtgc agcggcagcg gcagcgtctg 120 gccaggaggc gtggaggggc ccagggatgg ccacccccac agggagtcag ggagggcctg 180 gggcgacagc ggaaaggtta agcgtcnaaa aggtcaagtg ctaccgtgga naaatcatct 240 gagggggagg ctcccggtgg gacagtcacc aanaactgtn acacacaagg ggaaggggga 300 gggctttcct gtcacaaana ttaaaaaccc ccnaaatgca tttgaacaac atnatacacn 360 ataacaaatt taaaccttgc tcctctgtcc cactgggtna accctggccc atcccccatc 420 cctggtccca tcccaggggc ccagcctccg atnactcctc anaaacacng ccttnntgct 480 ggggggctgc tgtntncctg ccacccccnn gaaaaggtgc tgg 523 14 530 DNA Homo sapiens Unsure (37)..(37) n = a, c, g or t 14 aaaaccaact tttattgaaa aatttggagg gaaggtncca tnttatntta taatantaaa 60 aatactaaat ttaaattttn taaaaaaacc ccntgntgca ccggcancgg cancttctgg 120 ccaaaangct tnaaggggcc cagggatngc cncccccnca gggattcngg gagggcctgg 180 ggcaanancg naaaggttaa ccntcnaaaa ggtcaattnc taccgtgnaa aaatnatctn 240 agggggangc tcccggtggg acactccccn aaaactntna cccaaaaggg gaagggggag 300 ggctttcctn tnncaaaaat tnaaancccc cnaaatgcct ttnaacnact ttntncccan 360 tnncaatttt naaccttgcn cctctntccc actgggtnaa ccctggccca tcccccatcc 420 ctggtcccnt cccnggggcc caccccccna taacttcctc aaaaaccngc cttnttnctg 480 gggggctgct nttttcttcc ccccccaana aaaggtnctg gcccccctcc 530 15 311 DNA Homo sapiens Unsure (4)..(4) n = a, c, g or t 15 gccnancagg nanccgccgc tgaagccacc gccgggtgcc cagcgccgcc gccgcccccg 60 agctcccccg cgcccctgcc cncgggcggn cggtgggcac cgggcgccat ggccgcgccg 120 gganccgctg cggntncgcn tgtgccnctt ggtgcncgga anancanggc tacngnttct 180 acctntacgt gtgananngg ccgccgcggg cacttcntcc ggcgcgtgna ncctctgttc 240 ccccgccgag gcngccgcgc tgtgctctgg ggatctnctg ntcnaggtca acntgcntca 300 acgtgnaggg c 311 16 289 DNA Homo sapiens Unsure (4)..(4) n = a, c, g or t 16 gcanagaaag gtttgtttta ttgcaattat ttaaatcncg tcccangggg gaggggaagg 60 gggangggaa ggggggggtn tctggtnttn attngatncc tgtctgccan cttnnacatc 120 tatnangaan anaaccatca ncncncntcc ctttcantca tctggcncct gcanaccatc 180 tttcgccctc tnccccccgc tgctctccna ctcccntgac cnctctcatc tctctccnct 240 ctgnctcctc nctctntctc tcatttctct gttncacnct ctctccccc 289 17 356 DNA Homo sapiens Unsure (98)..(98) n = a, c, g or t 17 ctgaagccac cgccgggtgc ccagcgccgc cgccgccccc gagctccccc gcgcccctgc 60 ccgcgggcgg ccggtgggca tcgggcgcca tggccgcncc gganccgctg cggccgcgcc 120 tgtgccgctt ggtgcgcgga nagcanggct acggcttcca cctgcacggt gagaanggcc 180 gccgcgggca nttcatccgg cgcgtggaac ccggttcccc cgccgaggcc nccgcnctgc 240 gcgctgggga ccgcntgntc naggtcnacn gcgtcaacnt ggagggcgat accaccncct 300 ngtgntgcnt acgatcnang ctgtngangg gcanactcgg ctgctggtgg tggacc 356 18 290 DNA Homo sapiens Unsure (9)..(9) n = a, c, g or t 18 gcaaagaang gtttgtntta ttgcaattat ttanagcgcg tcccaagggg gaggggangg 60 gggangggaa ggggggggtn tcttgctana aactggaaac ntgtttctta ccccnatntc 120 nnantcgact nccaccaact gtnnntcttc cttcctttcc cnangtccct anntaccncc 180 tnttgccctt ctnccccttn tttcccctcn cgctttccct nactctttat ctntcttntc 240 ctctctctct ctcacctctt tctccccctc ccttcacnct cacnttgtct 290 19 714 DNA Homo sapiens Unsure (243)..(243) n = a, c, g or t 19 cgccagaaaa agttatttta attttctatt aaacattctt ctcaaagcat tattttatcc 60 tatatctcac tgaattttaa gaaataacat tagtattaga aaaactagga aaaaagataa 120 atgcagataa ttaaacttac atgaaaaagg aaaattataa caaaggactg agaacgttat 180 aaattgaaat gagattataa tttgaaaact gcatctgaaa gcaaacttta ttgttcaatt 240 atncttaatg atggtgtttt atgactaata cactgatttt tcaagaagga aacccatgtt 300 aaaaatattt ttattttaaa aataagcctg tgttcaagct ctgatcatat ttcttttatt 360 ttgatttggg aanaaaatac tgtttctgat agcatgaaat gcaaaatttt tagattttta 420 atctcactaa ttttaanaac tattgagaaa ttgattaatg acatgaagtg cacaacacta 480 attactggcc agctgttggc attgtgtttc ttacttagtt ctcccaaggg aaaactctta 540 aattgaatct tcagcagaat aatccttaaa tatactttgt aagcaaaaca aaagcttttt 600 tgtttacata gttctttggg attttactgt tcctaatttt attctgaaac tcaattttac 660 cccagaccat aattaccata ttaactttgt tntgcacagt tgtttgccaa ttca 714 20 687 DNA Homo sapiens Unsure (67)..(67) n = a, c, g or t 20 attttaattt tctattaaac attcttctca aagcattatt ttatcctata tctcactgaa 60 ttttaanaaa taacattagt attagaaaaa ctaggaaaaa agatnaatgc agataattaa 120 acttacatga aaaaggaaaa ttataacaaa ggactgagaa cgttataaat tgaaatgaga 180 ttataatttg aaaactgcat ctgaaagcaa actttattgt tcaattattc ttaatgatgg 240 tgttttatga ctaatacact gatttttcaa taaggaaacc catgttaaaa atatttttat 300 tttaaaaata agcctgtgtt caagctctga tcatatttct tttattttga tttgggaaga 360 aaatactgtt tctgatagca tgaaatgcaa aatttttaga tttttaatct cnctaatttt 420 aagaactatt gagaaattga ttaatgacat gaagtgcaca acactaatta ctggccagct 480 gttggcattg tgtttcttac ttagttctcc caaggaaaac tcttaaactg aatcttcagc 540 ngaataacct taaatatact ttgttagcca aacaaaactt ttttgtttac atagttcttt 600 ggattttact gttcctaatt ttattctgaa actccatttt tccccagacc ataattaccc 660 tatttaactt tgttatgcac agttgtt 687 21 994 DNA Homo sapiens Unsure (705)..(705) n = a, c, g or t 21 ctcacccagt tgctcctcag atgtttgggt atgctggaaa agaacatatg gaaaaatatg 60 gaacaaaaat tgaacacttt gcaaaaattg gatggaaaaa tcataaacat tcagttaata 120 acccgtattc ccagttccaa gatgaataca gtttagatga agtgatggca tctaaagaag 180 tttttgattt tttgactatc ttacaatgtt gtcccacttc agatggtgct gcagcagcaa 240 ttttggccag tgaagcattt gtacagaagt atggcctgca atccaaagct gtggaaattt 300 tggcacaaga aatgatgact gatttgccaa gctcgtttga agaaaaaagc attattaaaa 360 tggttggctt tgatatgagt aaagaagctg caagaaaatg ctatgagaaa tctggcctga 420 caccaaatga tattgacgta atagaacttc acgattgctt ttctaccaac gaactcctta 480 cttatgaagc actgggactc tgtccagaag gacaaggtgc aacgctggtt gatagaggag 540 ataatacata tggaggaaag tgggtcataa atcctagtgg tggactgatt tcaaagggac 600 acccactagg cgctacaggt cttgctcagt gtgcagaact ctgctggcag ctgagagggg 660 aagccggaaa agaggcaaag ttcctggtgc aaaggtggct ctgcngcata atttangcat 720 tggaggaact gtggttgtaa cactctacaa gatggggttt tcccggaagc cgccagttcc 780 ttttagaact catcaaaatt gaagccngtt ccaaccaagc tctgcaagtn atnggtttaa 840 ngnaaaatct ngttttaaag gnggattgag aaggaaacnt naaagaggga anggggaaca 900 atttgtgaaa gaaaaatncg gngggaattt ttgcccttca aggggaaana atggccctgg 960 ggggtaaaag anggccaccc tggggtggtg ggat 994 22 843 DNA Homo sapiens Unsure (12)..(12) n = a, c, g or t 22 ggccaaaaaa anttatttna atttcctatt aancntcctc cncaaancat tatttnaccc 60 tatnncncnc ngantttnan aaantacctt tnntnttaaa aaacctngga aaaaaaataa 120 tngcaaatan ttaaccttnc ttgaaaangg aaatttntac caanggacng aaancnttnt 180 aattngaant naaattatan ttngaaancg gcnncngaaa ccaancttna tggtccaatt 240 atcctnaang agggnntttn annactaatn cccngatttt ccaatangga ancccnnntt 300 aaaantnttt tnattttaaa aataacccng tntccaaccc cngatcanat tcctttnatt 360 tggattgggg aaaaaaatnc ngttccnnat accnngaann gcaaantttt taaattttta 420 acccccctan ttttaaaanc tatngaaaan tngattanng acttgaattg ccaaccctan 480 ttncnggcca ccngtgggcn tngtnttcct tacttantcc ccccaaggaa annccttaan 540 cngaanctcc nccaaaataa cccttaanta tccttggtaa ccaaancaaa acctttttng 600 tttacntant ccttgggatt taacgggtcc ccaatttnat ccngaaccca nttttccccc 660 naaccatant taccatttta ccttggtaag gcncagtngt ttgcantncc gcaaancagt 720 antnttcccc nggcnctttc ccccgancct tgggaaaaac gggatnggtc ccccccttaa 780 aaaacaacct tcccccncct ttggcccagg nnttnttccc gtctaaatcc gaacaataaa 840 aag 843 23 707 DNA Homo sapiens Unsure (99)..(99) n = a, c, g or t 23 ctagtctcga gttttttttt ttttttttaa cctttcctta tgagcatgcc tgtgttgggt 60 tgacagtgag ggtaataatg acttgttggt tgattgtana tattgggctg ttaattgtca 120 gttcagtgtt ttaatctgac gcaggcttat gcggaggana atgttttcat gttacttata 180 ctaacattag ttcttctata gggtgataga ttggtccaat tgggtgtgag gagttcagtt 240 atatgtttgg gattttttag gtantgggtg ttgagcttga acgctttctt aattggtggc 300 tgcttttagg cctactatgg gtgttaaatt ttttactctc tctacaaggt tttttcctag 360 tgtccaaana gctgttcctc tttggactaa cagttaaatt tacaagggga tttagagggt 420 tctgtgggca aatttaaagt tgaactaaga ttctatcttg gacaaccagc tatcaccagg 480 ctcggtaggt ttgtcgcctc tacctataaa tcttcccact attttgctac atagacgggg 540 tgtgctcttt tanctgttct tangtanctc gtctggtttc gggggtctta gctttggctc 600 tccttgcaaa gttatttcta agttnaattc attatgcnca angtataggg gttagtcctt 660 gctcatatta tgcttggtta taattttcca nctttcccct tgcggta 707 24 663 DNA Homo sapiens Unsure (133)..(133) n = a, c, g or t 24 ccattttata atgcgcttta tttgattaaa gaatttgcct tctttgtata cactggaatg 60 ttatattccc tatgtatttt acagggttac aaaatgtctc tcattttaaa tattacccca 120 aaagtaatct canaaaaaaa aggttttttg aaattaaact tgacttttaa aaaatcatac 180 ggacaaacaa ctttcaaaca aaactggatt agtaggattt cttgcctgct taactaacat 240 gacanacttc ttgtcccagg cccttctcan aaaaacctca tgtggaaacc aagctanaga 300 taanaattct tccctgatgc agttagggga aagggaaagg ctagaaactt ctttggcaag 360 caattccaca cacagccatt tatgtgtgag tgctctgctt caagcacagt acgctctttg 420 cagggacggc cagatgttca gagtgggagt ggtacttttc aaccagctaa aagtgcagaa 480 gtcatctant cgtctgcctc ttcccactgc cagtgcctgc agccttgcag caacttttaa 540 ccacccccta tgggactgga atnttgagtt aaaaagccaa ngctgaactg gctgacgctg 600 tantctccan tgaaaaggaa atgggatgaa atggaaaccg aaaaaccccc ngtnacntga 660 tga 663 25 621 DNA Homo sapiens Unsure (132)..(132) n = a, c, g or t 25 cattttataa tgcgctttat ttgattaaag aatttgcctt ctttgtatac actggaatgt 60 tatattccct atgtatttta cagggttaca aaatgtctct cattttaaat attaccccaa 120 aagtaatctc anaaaaaaaa ggttttttga aattaaactt gacttttaaa aaatcatacg 180 gacaaacaac tttcaaacaa aactggatta gtaggatttc ttgcctgctt aactaacatg 240 acaaacttct tgtcccaggc ccttctcana aaaacctcat gtggaaacca agctananat 300 aanaattctt ccctgatgca gttaggggaa agggaaaggc tagaaacttc tttggcaagc 360 aattccacnc acagccattt atgtgtgagt gctctgcttc aagcacanta cgctctttgc 420 agggacggcc anatgttcnn antgggagtg gtacttttca accagctaaa antgcanaag 480 tcatctantc gtctgcctct tcccactgcc agttgcctgc agccttgcag catcttttaa 540 ccacccctat nggactggaa tattgaatta taaacccngg ntgaactggc tgangctgtt 600 tctcccttga aaaggaaatg g 621 26 637 DNA Homo sapiens Unsure (6)..(6) n = a, c, g or t 26 catttnataa tgcgctttat ntgattaaan aatnngcctt ctttgtatac gcnggattgt 60 tatctcccct ntntatttnn gggggttaca anttntcnct catttnaant atnnccccaa 120 tantntnctn aaaaaaaaga ggtttganga aattaaactt gacttttaaa anatcatgng 180 gacaaacnac tttcaaacaa agctggatta gnaggatttc tngnctgctt aactaacatn 240 aaanacttct tgtcccaggc cctnctnaaa aaaacctctt gtggaaaccn agcnaaaaat 300 aananttctc ccctgatgca ntggggggag anggagaggc taaaaacttc tntggcaanc 360 anttccacnc acngccattt ttntntnagt gcnctgctnc nancnnagta cgctctttgg 420 gnggacggcn anntnttnat agngggagtg gtnctttcaa ccagctaata ntgaagaaat 480 catctagtcg nctgcctctn cccactgcca gtgcctgcnt ccttgcaacn tcttttaacc 540 ccccctangg acnggattat nnagttaana ccgaggntga gctggntgac gctntctcct 600 ccatttgaaa angaaatgga taagatggaa ccgaaaa 637 27 673 DNA Homo sapiens Unsure (659)..(659) n = a, c, g or t 27 agattatgcc attgaggcta agaatagagt catttttgat ctaatttatg aatacgaaag 60 aaagagatat gaagatcttc ctataaatag caatccagtg tcttctcaga aacaaccagc 120 cttgaaggct acaagtggca aggaagattc tatttcaaat atagccacag aaataaagga 180 tggacaaaaa tctgggacag tgtcttctca gaaacaaccg gccttgaagg atacaagtga 240 caaggatgat tctgtttcga acacagccac agaaataaaa gatgaacaaa aatctgggac 300 agtgcttcct gctgttgaac agtgtttaaa caggagtctc tacagacctg atgctgttgc 360 acagcctgtg acagagaatg agttttcttt ggaatctgag attatttcaa aactatacat 420 cccaaagaga aagattattt ctccacgatc tataaaagat gtgcttcctc ctgttgaaga 480 ggctgttgac aggtgtctct acctactgga ccgttttgca cagcctgtga caaagggata 540 agtttgcttt ggaatctgag aatatttcag aaccatactt tacgaacaga aggactattc 600 tcaacaatct gcagaaaatt tagatgctgc atgtggcatt gacaaaacag aaaatggana 660 catgtttgaa gac 673 28 714 DNA Homo sapiens Unsure (67)..(67) n = a, c, g or t 28 cctctggcta tattcaaaac agaatctttc tcatcacttg aagccttcaa gcctggtggt 60 ttctcanaan acactgtcct agatttttct ccatccttgt tttctctggc tatacccaaa 120 acagaatctt cctcgtcact tgtacccttc aaggttggtg gtttctgana anacactttc 180 ctanatattt ctccatcctt ttttcctctg gttatattcg aaaaanaatc cttctcatca 240 cttgtagcct tcgaggctgg ttttttccga naagacactg tcctanattt ttctccatcc 300 ttgttttctc tggctatact caaaacagaa ccttcctcgt cacttgtanc cgtcaaggct 360 ggtggtttct ganaanacac tgtcccanat ttttctccat cctttatttc tgtggctatg 420 ttcgaaacag aatctttctc atcagttgta gccttcaagg ntggttgttt ctgaaaanan 480 ctgtcccana tttttctcca tcctttattt ctgtggctat nttcgaaaca gaatcttcct 540 cgtcagttgt accttcnagg ntggttgttt ctgaaaaaan actgtcccac actgtatcca 600 tccttttatt tntgttanct atatcnaagc aaaatctgtt ttgtcccttg ttaccntttg 660 aaggtnggtn gtttctgaaa aataanctgt tccanatttt cccaccaccc attt 714 29 688 DNA Homo sapiens Unsure (67)..(67) n = a, c, g or t 29 cctctggcta tattcaaaac agaatctttc tcgtcacttg tagccttcaa gcctgatggt 60 ttctcanaan acactgttct anatttttct ccatcctttt tttctctggc tatattcaaa 120 acanaatctt cctcgtcacc tgtagccttc aaggctggtg gtttctgaaa anacactgtc 180 ctanatgttt ctccatcctt tctttctctg gttatatttg aaaaanaatc tttctcatca 240 cttgtagcct tcaaggctgc ttttttccga naanacactt caagcctggt ggttgctctg 300 aaaacactgt tctaaatttt tctccatcct ttttttctct ggctatattc aaaacanaat 360 cttcctcgtc acttgtagcc ttcaaggctg gtggtttctg aaaananact gtcctanatg 420 tttctccatc ctttctttct ctggttatat ttgaaaaana atctttctca tcacttgtan 480 ccttcaaggn tgcttttttc cganaanaaa cttcaagcct ggtggttgct cngaaaaaac 540 tgtcctaaaa tttttctcca tccttttctt ctctnggcta tactcnaaac aaaatcntcc 600 tcgtcccttg ttncccttca anggtgggtg gtttctcgaa aaaaanactg tcctanaatt 660 ttcctccntc cctttttttc tctgggtt 688 30 721 DNA Homo sapiens Unsure (264)..(264) n = a, c, g or t 30 ttcgggcttc cacctcattt ttttcgcttt gcccattctg tttcagccag tcgccaagaa 60 tcatgaaagt cgccagtggc agcaccgcca ccgccgccgc gggccccagc tgcgcgctga 120 aggccggcaa gacagcgagc ggtgcgggcg aggtggtgcg ctgtctgtct gagcagagcg 180 tggccatctc gcgctgcgcc gggggcgccg gggcgcgcct gcctgccctg ctggacgagc 240 agcaggtaaa cgtgctgctc tacnacatga acggctgtta ctcacgcctc aaggagctgg 300 tgcccaccct gccccagaac cgcaaggtga gcaaggtgga gattctccag cacgtcatcg 360 actacatcag ggaccttcag ttggagctga actcggaatc cgaagttgga acccccgggg 420 gccgagggct gccggtccgg gctccgctca gcaccctcaa cggcgagatc agcgccctga 480 cggccgangt gagatccaga tccgaccact anatcatcct tataccgacg gggaaacnga 540 agccatanaa ggcgtgggcg cttgcaccac ttccgtccca tccttgcggg tacctggtct 600 atgcnggggt ncctaaggac cttggaaaaa acgctccccc gtcgttgctt cctggggaan 660 ggggcgttnc gctgcgcttc ggaacggggt tccttccaac ccgccggtct catttcttct 720 c 721 31 461 DNA Homo sapiens Unsure (7)..(7) n = a, c, g or t 31 ccaccancta anttattnnt ttaataacaa aaaaacancc ccacaaaact atngtaaaac 60 aatatttcca ntcggtnatc ntngtattnt acaatacaaa ncanttcccn caaaattctn 120 aaaancacca ancttnacca ttttttaaan tttctgcttt ncaaaaanta aaaacncnca 180 attgnantcc caccccctaa attctctggt nactattagg tntncaaaaa gnaccncccn 240 ctccncncca ttgcctcanc cncancccca ggctgnatnc atttaagggc ncattggccg 300 ccaatcggnc tnntccnccc ncaaatccgg caaggcnctt nggggnaaac ccacaaanca 360 cttattcccc ctngccccct gaatggctgg ggtccgccgg tccctggggn aggcnctcca 420 ccaacncaaa atgcaatcnt ccncagnaac ccntgccgcc t 461 32 751 DNA Homo sapiens Unsure (239)..(239) n = a, c, g or t 32 cccgagggac cacagctggc agctccgggg atgccctcgg caaagcactg gcctcgatct 60 actccccgga tcactcaagc aataacttct cgtccagccc ttctaccccc gtgggctccc 120 cccagggcct ggcaggaacg tcacagtggc ctcgagcagg agcccccggt gccttatcgc 180 ccatctacga cgggggtctc cacggcctgc agagtaagat agaagaccac ctggacgang 240 ccatccacgt gctccgcagc cacnccgtgg gcacagccgg cgacatgcac acgctgctgc 300 ctggccacgg ggcgctggcc tcaggtttca ccggccccat gtcactgggc gggcggcacg 360 caggcctggt tggaggcagc caccccgagg acggcctcgc aggcagcacc agcctcatgc 420 acaaccacgc ggccctcccc agccagccag gcaccctccc tgacctgtct cggcctcccg 480 actcctacag tgggctaggg cgancaggtg ccacngcggc cgccancgag atcaagcggg 540 aagagaagga ngacgangag aacacgtcag cggctganca ctcggaagaa ganaanaagg 600 aactgaaggc ccccgggccc ggaccattac ggaacaagtg ctgtcccttg naggagaaaa 660 actgaaggac cgggaaaagg cncatggcaa ttacnccccg ggaaccggtg cccttccggg 720 atattaacna aggcttccgg gaactggggg c 751 33 556 DNA Homo sapiens Unsure (73)..(73) n = a, c, g or t 33 aatacaacgt ttaatcatct ggttgatcaa aaaatgcaat gctcagtcta ggaacagcag 60 caaaaatagc ganagacacg ggacttttat acaaaaaaat ttgttgctta caaaacatat 120 gcaaaaaaag cttaaaaaaa ccaaaaacca aaggcagcat ccttgctaat tttcatctac 180 attaanaaaa aaaaaatctt gtaactaatg tttttatttn ccttaaaaaa aatatttcgc 240 ttaggcacaa tttgctggtg gctttaaaaa aataagccag gtttccacag catccccctt 300 gagtgatatn tttccatttc tccgcttttt atagttaagg cattttttnc tnctctgaca 360 aagtgtatgt tttgttgctt gctttcaggt tttgtttact ggaaaaaaaa aaaaatgccc 420 tgtcanccca ngcaanaggg ccaanatgca attcagggat ccntgggaca ggtccaaaat 480 gacccggggg ctgaaattcc gggacggggg aacaaggcnn tttaatngta ggccagggcc 540 canggaaccc tgaacc 556 34 708 DNA Homo sapiens Unsure (7)..(7) n = a, c, g or t 34 ccacttnaat tcctttatnc ancaatatta tccnaaaagg aaaaatcagg atttacaaaa 60 acaatttaan tgcaatataa aaccctacta aatacaaata caattncaca aacncntatg 120 caacaaaaac ttgtttaaat ngttccttna atttnnacta cttaaaanca taggtntaaa 180 ggaaaaacnt ncaaactggt ccacttgggc ttnttaccag gcaaagnaac cctgcttncc 240 aaaaactnat atattccaaa ttcncggcat ntggnaatnt tnccatggac nctgnatctt 300 aacaaatgct atantnttta caaaactacn cccncaaaaa aaccccaagg aacctgcagg 360 ctaancccta tncttttaaa gggctnaagg aaccaaacct attttaancc tnttngtttg 420 cnccatgcaa aactttatgn aaaaccccca aactaggcta tttancnnct nccatnaatg 480 gnccccaaat catntnatnc tacggcataa acaacanctg ccctatttac ncggaacctg 540 caaanctcac aagnaatgtg aattngcnct ngggantcaa tgttnccggg tnaattatct 600 tggatnanaa ccnttttcta catnactatt gaaaaaacct gtggtttctt gctttttaac 660 aaatnnggtg ttcctttgcc cccccccctt atttttcaag ggctgggt 708 35 675 DNA Homo sapiens Unsure (43)..(43) n = a, c, g or t 35 atcacagtcc agagagtcct agaggaggac gagagcataa gancttgcct tagtgaagat 60 gcaaaagaga ttcagaacan aatagaggta gaagcagatg ggcnaacaga agagattttg 120 gattctcaaa acttaaattc aagaaggagc cctgtcccag ctcaaatagc tataactgta 180 ccaaagacnt ggaagaaacc aaaagatcgg acccgancca ctgaagagat gttagaggca 240 gaattggagc ttanagctga agaggagctt tccattgaca aagtacttga atctgancaa 300 gatntaatga gccaggggtt tcatcctgaa agagacccct ctgacctana aaaagtgaaa 360 gctgtggaag aaantggaga anaagctgag ccagtncgta ntggtgctga gagtgtctct 420 gagggtgaag gantagatgc tacttcaggc tccncagata gttctggtga tggggttacn 480 tntccatttn aaccngaatc ctggaagcct actgatnctg aaggtntgan gcnntntgac 540 ngggagttct gctggacttc cagttcatgc ctgcctggta tnctttnccc gagggcctgc 600 ctcctntcag tgatttggtt cttgacaaga tccnccntcc cccttttgcc aatgccgaac 660 tctgggatcc ttcga 675 36 713 DNA Homo sapiens Unsure (238)..(238) n = a, c, g or t 36 gctaaacatt tttttaagta tgagtccttg tttaaaaaga aaagattaaa acagaaaata 60 ttttctataa ataatacatg tattttggtt ttagtgctcc cgccctaagg tttgaagttt 120 acttttatcc agtacctttt tcctccatga tcaccttttt ttctctttcc cctctcccac 180 tcgtgcacac gtgggggttt ctgcgagaat tggccttgct gcactgtgat tggcgaanac 240 gtgaaacttt ttaaaaaaat acttaaattg tttcttttgt ttcattttgt gtatttgaag 300 ttttagttat cctcagactc ctcttctgct tcccgcagcc acgtgaagaa tgccgtgaca 360 gatttcagag ccacgccctt cccattctgc tctgcagggt ccttgctgct ctcccatttg 420 tagaaggcat cctcggagat cacctcctcg tcatatagac aatcaaaaaa catccgcagc 480 aaattggcag gttgatcaag ttttactatc gatgcttgta gtgcataaag tgctgcagtt 540 ccttctctgt atctgantct aggtacttga gtaagatcgg cactctctgc ttgataacag 600 cagtgtccac tctgaaggta naagaatcng gttattatag cttgctttaa caaacagcng 660 tcnttaaagc tctaaggaat gttangtgaa atncactgga tttcgtctaa att 713 37 554 DNA Homo sapiens Unsure (6)..(6) n = a, c, g or t 37 cattcnagaa agatnttaca cacggagttt nctcantatt gggctcaacg ggaagctgac 60 tttacggana ctctgcttca agtaacgana gatattanaa ganaatgctg gancgtcgtt 120 tggctatctt cnaggatttg gttggtaaat gtgaccctcg agaanaagca gcgaaagaca 180 tttntgccac caaagttgaa actgaagaag ctactgcttg tttagaacta actttnatcc 240 aattaaagct gaattagcta aaaccaatgg agaattaatc tcnaccncnc acnanttcnc 300 ccagaganaa natgaatccg attcattgat tcaagagctt gagacatctg ntaaganaat 360 aattncacan aatctggaga attnnagaat tgatnaatat nattgatcnn tcgaagatac 420 tatcancgaa tttcagaacc tnangtctca tatggaaaac tcntttaaat gcnatgacaa 480 ggctgataca tcttctttaa taataaacaa taaattgatt tgttatgaaa cagttgaagt 540 acctaaggga cagc 554 38 551 DNA Homo sapiens Unsure (65)..(65) n = a, c, g or t 38 gactgcatgt tctcatttat ttatgggatc taaaaataaa atcaattgac ctcatgggca 60 tacanantaa aaaaatggtt accagtggct ggtaagggta ctgacggttg cagggggagg 120 tggggatggt taatgggtac aaaaacaaat aagatnaaaa gaatgattta atatctgata 180 gcacaatana ntgactataa tcaataataa cttacttgta tatttttaaa tgatctaaaa 240 aatgtaattg gattatctgt aattcaaagg aaaaatgctt gaggggatgg atacctcatt 300 ctccatgata cacgtntttc acattgatgc ctgtgtcaaa acatctcaca taccccgtaa 360 atatatacat gtactatgta ccacaaaatg tttacaaaat aagtganaca ttctaattaa 420 agactgaaat ctttttctaa ataatgtata tacatgtttt gtgatctgta cacacttatt 480 ctccaaatcc taactntant cccaacanat atnttaaatc cttgtttanc ngaataagtt 540 aaaaaaatcc t 551 39 680 DNA Homo sapiens Unsure (125)..(125) n = a, c, g or t 39 atttggtaac aggattaaaa agaaattttt aattccttgt ctctcttctg atggctgaac 60 agaactgcgg tgtcaaatgg aaagcagcac acaagaattc ccttgcagac cttgatcttt 120 cgcanaaatg caaagacgcc tgagttatac aacttgcaat tattattttc tanacagaag 180 tgccaactgt tgtgctttcc agtgtatcag tggttgctac attctccttc ttgtcttcgg 240 gtttcatggc aggaaacaga agtacttcct tgatgttgtt ggagtccgtg agaaacatgg 300 cgactcgatc aatgcccatg ccccagccag ctgtgggggg cagcccatat tccagggcag 360 tacagaagtt ttcatctatg aacatggcct catcatcacc tgcagccttg gccttggcct 420 gttcttcaaa aanctgccgc tgccgcatgg gatcattcag ctcagtatac gcattgcata 480 tctctttctt catgacaaac agctcaaanc gctcagtcag acctctttaa ancggtgcca 540 tttaaccnaa gggccattat ctgtgggtga tcacagatga atgtnggatt gatgcaagtc 600 acttccanga actccccaac aancttgtca aggaacctgg ctgtggtcca angtggaagg 660 catccacanc ttttgccccc 680 40 753 DNA Homo sapiens Unsure (90)..(90) n = a, c, g or t 40 acaaatatga acgtctgaag gcaaaccagg tagctactgg cattcggtac aatgaaagga 60 aaggaaggtc tgaactaatt gtcgtggaan aaggaagtga accctcagaa cttataaagg 120 tcttagggga aaagccagag cttccagatg gaggtgatga tgatgacatt atancagaca 180 taagtaacag gaaaatggct aaactataca tggtttcaga tgcaagtggc tccatgagag 240 tgactgtggt ggcanaagaa aaccccttct cantggcaat gctgctgtct gaagaatgct 300 ttattttgga ccacggggct gccaaacaaa ttttcgtatg gaaaggtaaa gatgctaatc 360 cccaagagag gaaggctgca atgaagacag ctgaagaatt tctacagcaa atgaattatt 420 ccaagaatac ccaaattcaa gttcttccag aaggaggtga aacaccaatc ttcaaacagt 480 tttttaagga ctggagagat naacgatcag agtgatggct tcgggaaagt ttatgtcaca 540 gagaaagtgg ctcaantnna acnaattccc tttgatgcct cnnaattacn cagttctccg 600 cagatggcag cccagcacaa tatggtggat gatggttctg gccaagtgga aatttggcgt 660 gtncaaaaca atggtaggat ccaagttgac cnnaactcct atggtgactc ccatggtggt 720 gactgctact tcatactcta cacctatccc tga 753 41 675 DNA Homo sapiens Unsure (45)..(45) n = a, c, g or t 41 ggtgtttcca aaggctttta ataaggttaa aaaaaaaata aaatnccnct taaaaaataa 60 cncttancan ttaatgacat caaantcncn ttgactaaaa aaggaaaata ncaaccaatt 120 gttaaancca ccttaacata aaccttatng caattntaca cntcttttga acncaatcta 180 taaaaaaaaa aataactncc anggcattac aacttttnct ctggcatntt aaaaaacaac 240 tctnactaat ggctaatgca ttataaaatt ncctatctna caaatcttnc taaattatgc 300 atagtatttt acttttnaaa ggtcntaaaa aaaatataaa tcanttncca taaaanctaa 360 tatnggccca taacaaaant tccctncagg ttattttaat ntnttaacnt aaaaaaacnc 420 cagntgaaaa aaaattncaa nccaaaacta accnttaaaa aataggcntt nggttnaggt 480 taattttttt tttttttttt ttgnaaanaa antcnctntt gcccagnctg gattgtggtg 540 gcnccaatcc tggctcactg caacctcagc ctcctgggtt caagcaattt ncctgtctca 600 gccttccaan ttccngggaa tacaggggtn cnccaccacn cccagctaaa ttttttttgt 660 tttttttant aaaag 675 42 677 DNA Homo sapiens Unsure (460)..(460) n = a, c, g or t 42 aagatcagcg atatcacgcg tcccccggag catcgcgtgc aggagccatg gcgcgggagc 60 tataccacga agagttcgcc cgggcgggca agcaggcggg gctgcaggtc tggaggattg 120 agaagctgga gctggtgccc gtgccccaga gcgctcacgg cgacttctac gtcggggatg 180 cctacctggt gctgcacacg gccaagacga gccgaggctt cacctaccac ctgcacttct 240 ggctcggaaa ggagtgttcc caggatgaaa gcacagctgc tgccatcttc actgttcaga 300 tggatgacta tttgggtggc aagccagtgc agaatagaga acttcaagga tatgagtcta 360 atgactttgt tagctatttc aaaggcggtc tgaaatacaa ggctggaggc gtggcatctg 420 gattaaatca tgttcttacg aacgacctga cagccaagan gctcctacat gtgaagggtc 480 gtanagtggt gagagccaca gaattcccct tagctgggac agtttcaaca agggtgactg 540 cttcatcatt gaccttggca ccgaaattta tcanttggtg tggttcctcn tgcaacaaat 600 atgaacgtct gaaggcaaac cangtancta ctggcattcg gtncaatgaa aggaaaggaa 660 ggtctgaact aattgtc 677 43 665 DNA Homo sapiens Unsure (516)..(516) n = a, c, g or t 43 gagacagagt ctctgttgcc caggctggag tgtggtggcg caatcctggc tcactgcaac 60 ctcagcctcc tgggttcaag caattttcct gtctcagcct tccaagtagc agggactaca 120 ggcgtgcacc accacgcgca gctaattttt ttgtattttt agtaaaggcg aggtttcgcc 180 atgttggcca ggctggtctc gaaatcctga ccccagtgat ctgcctacct catcctctca 240 aagtgctggg attacaggtg tgagccaccg cgcccagcct taattttcaa aagacaaata 300 agcaaaaagc ttttcccgtt cctctcccaa aacagcaatg agataactgc cttgtaatgt 360 ttgtttgctt tttacaaata ccaatttacc acttgctgga atcccagccc aggaaccagc 420 ctgtgaatgt gggtggctca tggccctgtt ttatgatgac aattggtgtc ctcttgtctc 480 ttccagaagg gtctgtctca aggtacattt tggcanactt caaagattct tttttctcaa 540 cttcattagc atctttgcca atccaaataa atatctgttc ccaagcatct agtaacatga 600 catcatcttc agctaaatca tcctgggtga actctccctg gaatctcttc aataacaaat 660 ctccc 665 44 698 DNA Homo sapiens Unsure (180)..(180) n = a, c, g or t 44 ctgaggagct ggtggtcttt gaggatttga atgtatttca ctgccaggaa gaatgtgtga 60 gcttggatcc tactcaacaa ctcacgtcag agaaggaaga tgacagcagt gtcggggaaa 120 tgatgttact ggtcaatggc agtaatcctg aaggtgaaga tcctgagagg gaacctgtan 180 aaaatgaaga ttatagagaa aagtcttcag atgatgatga aatggattct tccttggtct 240 ctcagcagcc tcccgataac caggaaaagg aacgactaaa tacatccatt ccacaaaaaa 300 ggaaaatgag aaatctgtta gttaccattg agaatgatac tcctctagag gaactctcaa 360 aatatgtaga catcantatt attgccctta ctcgaaatcg gaggacaagg agatggtaca 420 cttgtccact gtgtgggaaa cagtttaatg aaagttctta cctcatttcc caccagagga 480 cccacactgg agaaaaaccc tatgactgtn ntcactgtgg gaaaagcttc aatcatnaaa 540 caaacctcaa taaacatgag cgaattcnta caggagagaa accttattcc tgttctcagt 600 gtggaaaaaa cttccgtcng aattctcatc ggagtcgtcc tgaaggaatc catntaacgg 660 agaagatatt aagtgtccan aatgtgggaa aacctccc 698 45 466 DNA Homo sapiens Unsure (203)..(203) n = a, c, g or t 45 atcacaaatt gtaaatatta ttgaaattga ttgcaaattt agatcacata caaatgagag 60 tctgacattc aactgttttc ctatattcca aagtaaacaa ttcctttcaa cactcaagac 120 ttaaacaggt attcttagag ggttatatga attgctatca gaagctgttg gctaacaagc 180 cagtaatttg gttctttcac canaacacag ttccagataa gcatctttgc actatttctc 240 aantatgaat ccccatgtgg ggggaaaacg gatatacttt caatagacac aagtcactct 300 ttgccttcca agtaagcana ctccagattc atcttcaaag tgttgggaaa ngggatctgt 360 gacctgtnca ttatcatata acttcaaaaa ggaaagctcc ttantccaaa aagcctanat 420 gctgaggtat agcccttgaa atgttttctt ccctgtnaat ttccta 466 46 734 DNA Homo sapiens Unsure (168)..(168) n = a, c, g or t 46 cacatacagt ctttgtttta atgtttattg gtagaaacag atcttcaatg catactttgt 60 gtttatataa actctacatt ctcttaaagg ttttcgtttt gttttcactg gagattttta 120 gcctccaagt gaacttaaca tattgcctat gcatctgatt ctttatanac ttttanattt 180 taaaactaaa tttganaaac catgcatact gtatacctta tttaataatc caaanaattg 240 tttgcacttt caaaaaagtt acaaaaaggc tgaacacaag ttaaataacc tatatgatgt 300 aaattttcca tttctgaata ctttttcagt attatatatt gcttgctgtc taataagtta 360 gattgtcaga nacgcttcag taaattatct ctactttaaa attatatctg aatccccttt 420 ctctganatg aacttgccaa tattaaacat tgtgccatat gcagtattan cccaaaagct 480 taaataagaa ccaaacttgt agactgaata ttttaacctt aaaattatat acctatatat 540 ncacctatgg tatgctgcat attaaattta acatttcaag taacatatat atagcaaaca 600 ttcagccaaa tactctttca tgaaaagata ctgtccttaa aataaaaagt tantgaaaag 660 cttatttaga ccnaatgtct aaatataant nctaagccta tgaaacttga anctaaagtc 720 tgctgtncta ttta 734 47 822 DNA Homo sapiens Unsure (709)..(709) n = a, c, g or t 47 ctcctgtgct tacacctgta gaaaaacacc agagcagaga gtatctcaag tgatgaagag 60 gttcatgaat ctgtggattc agacaatcag caaaataaaa aagttgaagg tggatatgaa 120 tgtaaatatt gtacttttca aactccagat ctaaatatgt ttacttttca tgtggattcg 180 gaacatccca atgtagtgct aaattcatcc tatgtttgtg tcgaatgcaa ttttcttacc 240 aaaaggtatg atgcactttc tgagcataat ctgaaatatc acccaggaga agagaatttt 300 aagttgacta tggtgaaacg taataaccag acaatctttg aacaaacaat aaatgatctg 360 acttttgatg gtagttttgt taaagaggag aatgcagagc aagcagaatc tacagaagtt 420 tcttcttcgg gaatatctat cagtaaaact cctatcatga aaatgatgaa aaataaagtg 480 gaaaataaac ggattgcagt tcatcataac tcagttgagg acgttcctga agagaaagag 540 aatgaaatca aaccagaccg tgaagaaatt gtagaaaatc caagttcttc agcttctgaa 600 tctaatacaa gtacttccat tgtaaacaga atacatccaa gtactgccag cacggtagtg 660 acccagcagc agttcctccc tggattgggc ccaggtgata actgctgtnt ctgctccgcc 720 agaattctaa tttgattccc naagtcttaa tccctgttna tancatcccc cctacaatgc 780 tgcnttggat aacaaccccc tttttactta acccctacan cc 822 48 819 DNA Homo sapiens Unsure (274)..(274) n = a, c, g or t 48 acccatttct aacaattttt actgtaaaat ttttggtcaa agttctaagc ttaatcacat 60 ctcaaagaat agaggcaata tatagcccat cttactagac atacagtatt aaactggact 120 gaatatgagg acaagctcta gtggtcatta aaccccctca gaaagtctaa gattcagaat 180 gtctccatca tattagaaga aaaatgtact gtattaaaat ttaaattgca tttttacaag 240 ttgtttttta attagtgttc tatttacatt gcanaacttc caccaactgc agtagtttaa 300 ctttggcaca acattaagtt ccatttcttt tgggtattgg atcctgcttt ttgagtgtgt 360 atgccccaaa acgttttcaa tgtcatcaaa gattgggcaa attcacagta aatcagacat 420 cttgagttga agaattgatt ctccttcaac gttttaggca gatttcagtc atctgattta 480 gacagcttcc gtttcacatg tcgtggaagt cccaagtgtc actatcatct gtttcttctt 540 catcctcttc ctggtcatca ataacttcat cttcctcctc attttcctca aataattcta 600 tacctaattc tgatcttctc tgtctttctg caaaccactc tctgacctgc tcatanccca 660 tatgtgattt gttaacaaat tcatcaaggt cttgctcatt aaaaaacttg tgcttcaggt 720 tataatcctt aanttttgcc gttccagttt taaattttat gaatnaatgg tcccctggtc 780 cccagttgtt aattcctttt ggctcctcca aggcgccca 819 49 704 DNA Homo sapiens Unsure (92)..(92) n = a, c, g or t 49 aaaaagttat ttatttattc tttttttttt tttttttttt ttggtaaggt tgaatgcact 60 tttggttttt ggtcatgttc ggttggtcaa anataaaaac taantttgan anatgaatgc 120 aaaggaaaaa aatattttcc aaantccatg tgaaattgtc tcccattttt tggcttttgg 180 gggggttcag tttgggttgc ttgtctgttt ccgggttggg gggaaagttg gttgggtggg 240 aggganccag gttgggatgg agggagttta caggaagcan acagggccaa cgtcnaagcc 300 naattcctgg tctggggcac caacgtccaa gggggccaca tcnatnatgg gcaggcggga 360 ggtcttggtg gttttgtatt caatcactgt cttgccccag gctccggtgt gactcgtgca 420 nccatcgaca gtgacgctgt aggtgaancg gctgttgccc tcggcgcgga tctcgatctc 480 gttggaaccc tggagganca gggccttctt gaggttgcca gtctgctggt ccatgtaggc 540 cacgctgttc ttgcantggt angtgatgtt ctgggagcct cggtggacat caggcgcagg 600 aaggtcacct ggatgccaca tcngcanggt cggaaccctg gccgccatac cccaactggg 660 aatccatcng tcatgctctc cccgaaacaa aacatcctct tgtt 704 50 693 DNA Homo sapiens Unsure (22)..(22) n = a, c, g or t 50 gaaagcaaat ttcttttaat ganaactcaa aattaaactt caaagggacc caacgtcata 60 cttccattca gggacttgat acaaaaaatt tagtttgaac tgctattagc aggtggcagg 120 agccaccttc aaatgaatct tcaaattgga aaatactgct tcaccacctg ttggggataa 180 nttgcaaatg gaataattta gtatggtttg tagctatttt gatnaccacc tcgcctgnat 240 accttcccat aaccactctg ctggtcacca cctcttccac aagctcttcc tgcaaatcct 300 cctctaaatc cccactgttg ctgttgctga tattgtncct tcgacatggc tacttttatt 360 tcacatttac taaaaccaac attgtggtat ttcttttcca ttatcttctt cactggttct 420 tcttccttaa aggtaataaa gcaaaaccca cgcctcttat tggtcttgtt gtccatgggg 480 agctctatgg attccacctc accaaaacca ccaaagtact cccttatttt ctcttcaggt 540 gtatctggan aaaggccacc ancnaaaatt ttttaaccgg ctcttttgtt tccatggctt 600 tgggcctttt angatcaatc accttcccca ttcaatttat gttctttttg gatccatgaa 660 cctttntcta cnccctcccg aattccttaa ata 693 51 619 DNA Homo sapiens Unsure (78)..(78) n = a, c, g or t 51 gcactctgaa gttagatcct atcacagggc gatcaagggg ttttggcttt gtgctattta 60 aagaatcgga gagtgtanat aaggtcatgg atcaaaaaga acataaattg aatgggaagg 120 tgattgatcc taaaagggcc aaagccatga aaacaaaaga gccggttaaa aaatttttgt 180 tggtggcctt tctccagata cacctgaaga gaaaataagg gagtactttg gtggttttgg 240 tgaggtggaa tccatagagc tccccatgga caacaagacc aataagaggc gtgggttctg 300 ctttattacc tttaaggaag aagaaccagt gaagaagata atggaaaaga aataccacaa 360 tgttggtctt agtaaatgtg aaataaaagt agccatgtcg aaggaacaat atcagcaaca 420 gcaacagtgg ggatctanag gaggatttgc angaagagct cgtggaagan gtggtggccc 480 cactcaaaac tggaaccang gatatantna ctattggaat cnaggctatg gcaactatgg 540 atatnacagc ccaggttacc gtggttntgg aagatatgac tncactggtt acnacaacta 600 ctatggatat ggtgattat 619 52 694 DNA Homo sapiens Unsure (27)..(27) n = a, c, g or t 52 cggcggcgcc attaaagcga ggaggangcg agagcggccg ccgctggtgc ttattctttt 60 ttagtgcagc gggagagagc gggagtgtgc gccgcgcgag agtgggaggc gaagggggca 120 ngccagggan aggcgcagga gcctttgcag ccacgcgcgc gccttccctg tcttgtgtgc 180 ttcgcgaggt acagcgggcg cgcggcancg gcggggatta ctttgctgct agtttcggtt 240 cgcggcagcg gcgggtgtat tctcggcggc agcggcggag acactatcac tatgtcggag 300 gancanttcg gcggggacgg ggcggcggca ncggcaacgg cggcggtagg cggctcggcg 360 ggcgaacang angganccat ggtggcggcg acacangggg cancggcggc ggcgggaacn 420 gaccgggacc gggggcggaa ccgcntctgg angctccnaa gggggcnncg ccnaatccga 480 aggggcgaaa attgaccccg tatgaaccaa gaagatgaat ggaaaatgtt tatangaagc 540 cttanctggg acactnccca gaaagatctg aaggactact tttccnaatt ttgggtgaaa 600 ttgttaaact gccctcttga aattttnatn ctatcccngg ggcnatcaaa ggggtttttg 660 gcttttttcc tattttaaac aaatcccgaa aaat 694 53 728 DNA Homo sapiens Unsure (10)..(10) n = a, c, g or t 53 cgccactgcn gcaggaggcg tgaggggata aaaacattca gatggcagat cacagttttt 60 cagatggggt tccttcagat tccgtggaag ctgctaaaaa tgcaagtaac acagaaaagc 120 tcacagatca ggtgatgcag aatcctcgag ttctggcagc tttacaggag cgacttgaca 180 atgtccctca caccccttcc agctacatcg aaactttacc taaagcagta aaaagaagaa 240 ttaatgcatt gaaacaactt caggtgagat gtgctcacat agaagccaag ttctatgaag 300 aggtacatga cttggaaaga aagtatgcag cgctatacca gcctctcttt gacaagagaa 360 gagaatttat caccggcgat gctgaaccaa cagatgcgga atcggaatgg cacagtgaaa 420 atgaagagga agagaaattg gctggagaca tgaaaagtaa agtagtcgtc acagaaaaag 480 cagcggcaac ggctgaagag ccagatccca naggaattcc agagttctgg tttaccatct 540 tcagaaatgt ggacatgctg agtgaattan tccaggaaat atgatgaacc aatcttgaaa 600 acacctgcag gatnttaaag ttgaaatttt ctgaccctgg acagcctatg tcttttgtgt 660 tagaattcca ctttgaaccc cacgactact ttaccaactc agtcctgaca aaaaccttac 720 cagatgaa 728 54 732 DNA Homo sapiens Unsure (255)..(255) n = a, c, g or t 54 acctaattag atagaagttc aggaatttct atttcttttg ggttgatgaa ccacaggcta 60 gcataagtcc actgtcaata aatgtttgtt gtggccagac ctccataaaa gagatattcc 120 ctgtgttcac aagttccctg aagcttaggt tttgagagaa tattgttgag tcactaggca 180 gggctcacat aggaaactgg caatcacctc tgaaactgct tcacagacac ctgcttttcc 240 tgctctgttc ctcanacttc tcctcttcaa gcgtattccc cccacaacaa ggacagcagc 300 ttggactaca tatctggctg atgatgtaat aaaaagatta ggcatggggg tttcctaagc 360 cacaattcag ggccactctg caccaacaga gataagcacc caggtggaag ccccccttcc 420 ccgagcctca tacattgtca tcatcttcta tggcctcccc agtgaagtac agcacagccc 480 gcgggactat ccgctcacgg aaaaagtgtc caatttcaaa atcagaagct aatgtgaatt 540 caaaatcttc atccagtgat ctccatcccc ggatgctttc caatggattg aagaaattga 600 aaaaggactc attgggtact gtttcgtaat tgttctaaca gtgcctcaac cttatgcttc 660 tgctttncnt ggaaggtntt gaaagtaaca ttcttncctt cttccaantc aattattnac 720 ccccgttcac aa 732 55 582 DNA Homo sapiens Unsure (121)..(121) n = a, c, g or t 55 gcttttcttc ttcctcctct tctgattctg gctcttgctt tatttgtggc tgcctgcgcc 60 tctcagcctc ttgttccatc ctctggagct ctgcatctgg atctggatgc ccttctgcca 120 naaggcgctg cctgatttcc tcaagctcct ccttctctct cttcctatct cgttcatctg 180 cttccatttc cttttctcta tcacgcaacc ttttctgaaa agcacttcct ctgtaatatt 240 tggggtcatc tctatcatca tcatagtctt ctaanaattc ttttagtcgt ttagcttctt 300 tggccatttc tcttcttctt tcttcttctc tttcagcttc tttctcatat tcccgggttt 360 tctttcgttc tctgatttcc caattcttaa ggcgctcttg ataancagct tctttctctc 420 gganttttct ttcaagtttt cttcgttcgt atgcatcttc ttcatcttct tctcggtccc 480 gttttttgtc tttttctctt tctcgctccc gttcctctct cnctctctct ctcgctcccg 540 ttctcgttct cgctctcntt ctctctctct ctctctttcc cg 582 56 784 DNA Homo sapiens Unsure (306)..(306) n = a, c, g or t 56 aagaaaagct caagtttcca agtctcttaa gggagcgatt aggcatgtca gctgatccag 60 ataatgagga tgcaacagat aaagttaata aagttggtga gatccatgtg aagacattag 120 aagaaattct tcttgaaaga gccagtcaga aacgtggaga attgcaaact aaactcaaga 180 cagaaggacc ttcaaaaact gatgattcta cttcagggag caagaagctc ctccactatc 240 cgtatcaaaa ccttctctga ggtcctggct gaaaaaaaac atcggcagca gggaagcaga 300 gagacnaaaa agcnaaaagg gatacaactt gcatcaagct aaagattgat agtgaaatta 360 aaaaaacagt agttttgcca cccattgttg ccagcagagg acaatcagag gagcctgcag 420 gtaaaacaaa gtctatgcag ggaggtgcac atcaagacgc tggaaganat taaactggag 480 anggcactga gggtgcagca gagctctgag agcagcacca gctccccgtc tcaacacgag 540 gccactccag ggggcnaggc ggctgctgcg aatcaccnnn agaaccggga tgaaagaaga 600 gaagancctt ccgggaaggg aatgaagttg attctcagag cngtattaga acngaagcta 660 aagangctcc gggtgagaac nccggggttg acctccctaa aattccagtc cagagatgtn 720 agacctgaaa gagaacccct gccganaccg ccgggaaagg ganaaatccg tcttgacccc 780 cttc 784 57 749 DNA Homo sapiens Unsure (141)..(141) n = a, c, g or t 57 cctacatcag ttttatttaa aacacaaaca agtatttctc tttctgtaag ggcaaatggt 60 tcaaataatg cggaacacga aacattgact aatacaagtg ctttaaatat gaaacaaaat 120 tattttttaa aaaagcaaaa naataaagaa tatatacaaa agggacctgn aatctgtaag 180 gtgattccaa aaacnaaata antagaaaat ccatggtgaa acctgaacat tctacctctg 240 ctttggagaa gggctatcat acaacattca gtcagctgaa natggattgg tanaggtgtg 300 tctatacata aacttcagtc atttttgctt gtgcanaatc atcccaatct tcccaanact 360 gaatgggcag tcctgtggct ttcttccttt tccatattcc caacaaggct acgtgaagtt 420 caactcttga tgagccgctt acaacagcag ttccttaggg agccaacatg acaggtgggt 480 canatttccc tatgagaaac aaaactggcc acctacagca aaatatcaaa atgggtaagt 540 ccttccttcc tcttcctcct gattatatac aacatatctc ctttcaagac tattatttcc 600 atcatgctta ttccttcaca aatctaaacc ttgaggtgat atgaaggaaa ccancntcaa 660 aaaaaagaaa actcaattcc gaaatgaana aaactgggcn nggtatncaa tacnccccan 720 aacatctcca tatccctggc ccagttacc 749 58 751 DNA Homo sapiens Unsure (364)..(364) n = a, c, g or t 58 agactttcct acatcagttt tatttaaaac acaaacaagt atttctcttt ctgtaagggc 60 aaatggttca aataatgcgg aacacgaaac attgactaat acaagtgctt taaatatgaa 120 acaaaattat tttttaaaaa agcaaaagaa taaagaatat atacaaaagg gacctggaat 180 ctgtaaggtg attccaaaaa cgaaataagt agaaaatcca tggtgaaacc tgaacattct 240 acctctgctt tggagaaggg ctatcataca acattcagtc agctgaagat ggattggtag 300 aggtgtgtct atacataaac ttcagtcatt tttgcttgtg cagaatcatc ccaatcttcc 360 caanactgaa tgggcagtcc tgtggctttc ttccttttcc atattcccaa caaggctacg 420 tgaagttcaa ctcttgatga gccgcttaca acagcagttc cttaggancc caacatgaca 480 ggtgggtcag atttccctat gagaaacaaa actggncacc tacagcaaaa tntcaaaatg 540 ggtaagtcct tccttcctct tcctcctgat tatntacaac atatctcctt tcaagantat 600 tatttccatc atgcttattc cttcccaaat ctaaaccttg aaggtgattt gaagggaaac 660 cnccatccnn aaaaagaaaa acccattccc aaattgaaaa aaaactnggc agggtataca 720 atacaccccc canaaactcn ccaattttcc c 751 59 760 DNA Homo sapiens Unsure (195)..(195) n = a, c, g or t 59 cctacatcag ttttatttaa aacacaaaca agtatttctc tttctgtaag ggcaaatggt 60 tcaaataatg cggaacacga aacattgact aatacaagtg ctttaaatat gaaacaaaat 120 tattttttaa aaaagcaaaa gaataaagaa tatatacaaa agggacctgg aatctgtaag 180 gtgattccaa aaacnaaata agtagaaaat ccatggtgaa acctgaacat tctacctctg 240 ctttggagaa gggctatcat acaacattca gtcagctgaa natggattgg tagaggtgtg 300 tctatacata aacttcagtc atttttgctt gtgcanaatc atcccaatct tcccaanact 360 gaatgggcag tcctgtggct ttcttccttt tccatattcc caacaaggct acgtgaagtt 420 caactcttga tgagccgctt acaacagcag ttccttagga gccaacatga caggtgggtc 480 aaatttccct atganaaaca aaactggcca cctacagcaa aatatcaaaa tgggtaantc 540 cttccttcct cttcctcctg attatataca acatatctcc tttcaagact attattccat 600 catgcttatt ccttcacaaa tctaaacctt gaagtgatat gaangaaacc nccntccaga 660 aaagaaaact cnantcanaa atgaaaaaaa ctggcaggta tncaatacac cccaaaacnt 720 ctcaatntcc tggcacanta caatccattg ttctgctaca 760 60 1041 DNA Homo sapiens Unsure (763)..(763) n = a, c, g or t 60 caaaggcgac agctgcccat tccgtcactg tgaagctgca ataggaaatg aaactgtttg 60 cacattatgg caagaagggc gctgttttcg acaggtgtgc aggtttcggc acatggagat 120 tgataaaaaa cgcagtgaaa ttccttgtta ttgggaaaat cagccaacag gatgtcaaaa 180 attaaactgc gctttccatc acaatagagg acgatatgtt gatggccttt tcctacctcc 240 gagcaaaact gtgttgccca ctgtgcctga gtcaccagaa gaggaagtga aggctagcca 300 actttcagtt cagcagaaca aattgtctgt ccagtccaat ccttcccctc agctgcggag 360 cgttatgaaa gtagaaagtt ccgaaaatgt tcctagcccc acgcatccac cagttgtaat 420 taatgctgca gatgatgatg aagatgatga tgatcagttt tctgaggaag gtgatgaaac 480 caaaacacct accctgcaac caactcctga agttcacaat ggattacgag tgacttctgt 540 ccggaaacct gcagtcaata taaagcaagg tgaatgtttg aattttggaa taaaaactct 600 tgaggaaatt aagtcaaaga aaatgaagga aaaatctaag aagcaaggtg agggttcttc 660 aggagtttcc aagtctttta ctccaccctg agcccgttcc aagtcctgaa aaagaaaatg 720 tcaggactgt ggtgaaggac agtaactctc tccaacaaac aanggagaaa gaanccttgg 780 gtagattgag tcctactgan agacggggga aacgaaaant tcagcaagcg gtgacaagtg 840 atcctccaat aaagcgttac cctgcacaaa ggctagggaa aaaaanttaa ancccanaaa 900 actaacattg acaaaaccac caaagaaagc tcaagnttcc aagtccccta agggaccgan 960 taagcatgtc aaccggatca anataatgng gntgcaacag ttaaagntta aaaaattggg 1020 gaaattcagt taaaacattt g 1041 61 810 DNA Homo sapiens Unsure (33)..(33) n = a, c, g or t 61 ccctacatca gttttattta aaacacaaac aantatttct ctttctgtaa gggcaaatgg 60 ttcaaataat gcggaacacn aaacattgac taatacaant gctttaaata tgaaacaaaa 120 ttatttttta aaaaagcaaa agaataaana atatatacaa aagggacctg naatctgtaa 180 gctgattcca aaaacnaaat aantanaaaa tccatggtga aacctgaaca ttctacctct 240 gctttggana agggctatca tacaacattc antcagctga aaatggattg gtaaaggtgt 300 gtctatacat aaacttcant catttttgct tgtgcaaaat catcccaatc ttcccaaaac 360 tgaatgggca gtcctgtggc tttcttcctt ttccatattc ccaacaaggc tacntgaant 420 tcaactcttg atnagccgct tacaacagca gttccttagg agccaacatg acaggtgggt 480 caaatttccc tatgaanaaa caaaactggc cacctacagc aaaatatcaa aatgggtaag 540 tccttccttc ctcttcctcc tgattatata caacatatct cctttcaaga ctattatttc 600 catcatgctt attccttcac aaatctaaac cttgaggtga tatgaaggaa accancatca 660 agaaaagaaa accaattcan aaatgaanaa aactggcagg tntacaatac accccananc 720 atctcaatat ccctggcaca gttacaattc agtgttctgc tacagcccat aaaataaata 780 ttggcagctt gaataancnc attttttccc 810 62 656 DNA Homo sapiens Unsure (4)..(4) n = a, c, g or t 62 cccnacatca nttttattta aaacacaaac aattatttct cttnctgtaa gggcaaatgg 60 ttcaaataat gcgnaacaca aaacnttgac taatacaatt gctttaaata tnaaacaaaa 120 ttatttttta aaaaancaaa aaaataaaaa atatntacaa aagggacctg aaatctgtaa 180 nctnatncca aaaacaaaat aattaaaaaa tccatggtna aacctnaacn tnctacctct 240 gcttnggaaa agggctatca tacaacntnc antcanctna aaatggatng gtaaaggtnt 300 ntctatacat aaacttcant cattttngct tgtgcaaaat cancccaatc tncccaaaac 360 tnaatgggca ntcctgtggc ttnctncctt tnccatatnc ccaacaaggc tacttnaatt 420 tcaactcttn ataanccgct tacaacagca ntnccttagn anccaacatn acaggtgggt 480 caaattcccc tataaaaaac aaaactggcc ncctacanca aaatatcaaa atgggtaatt 540 ccttcctncc tctnccncct nattatatac aacatttctc ctttcaaaac tattattncc 600 atcatgcttn ttcctncaca aatctaaacc ttgangtgat ttgaaggaac cacctc 656 63 645 DNA Homo sapiens Unsure (10)..(10) n = a, c, g or t 63 gaacttcccn acnncatttt tatttaaaac ncaaacaatt nttncncttn ctntangggc 60 aantggtnca aatantgcgn aacncaaaac tttnactaat acaattgctt taaatntaaa 120 ncaaanttat tttttaaaaa accaaaaaaa taaaaaatnt ttccaaangg gacctgaaan 180 ctntaaccta atcccaaaaa caaaataatt aaaaannccn nggtnaancc tnaacntnct 240 nccnctnctt tgnaaaaggg ctatcanaca acntncattc ncctaaaaat gnatnggtaa 300 aggtttttct anacataaac ttcattcatt ttggcttntn caaaancacc ccaanctncc 360 caaaactnaa tgggcnncct ntggcttnct ccctttccca tntncccaac aaggctactt 420 naattncaac ncttnataac ccccttacaa caccattncc ttagnaccaa cataacaggt 480 gggtcaaatt ncccnataaa aaacaaanct ggcccctncc ccaaaatncc caaatgggta 540 ttcctncctn ccctcccccc ngnatatata caacatntcc cctttcanaa atatattccc 600 ccacgcttat tccncccaaa nntaancctt gaagttattt aagga 645 64 780 DNA Homo sapiens Unsure (613)..(613) n = a, c, g or t 64 ccttgacctc ccaaagtgct gagattacag gcctgagcca ctgcaccttg ccttccttac 60 ctcttttctc cgacattttt atgtttctaa cattgaactc taaggaagct ggtgaacaaa 120 cacgccatat gtatgcagaa cacttaacag aattatgcta tgttgtctgt ttttgtttgt 180 atttcttgtc cttgctgaag attgacttga aatcttaaac taagttctcc ctctttatag 240 gcggtgacag tgatcctcca ttaaagcgta gcctggcaca gaggctaggg aagaaagttg 300 aagctccaga aactaacatt gacaaaacac caaagaaagc tcaagtttcc aagtctctta 360 aggagcgatt aggcatgtca gctgatccag ataatgagga tgcaacagat aaagttaata 420 aagttggtga gatccatgtg aagacattag aagaaattct tcttgaaaga gccagtcaga 480 aacgtggaga attgcaaact aaactcaaga cagaaggacc ttcaaaaact gatgattcta 540 cttcaggagc aagaagctcc tccactatcc gtatcaaaac cttctctgag gtcctggctg 600 aaaaaaaaca tcngcagcag ggaactgaag agacaaaaaa gccnaaagga tacaacttgc 660 atcaagctaa agattgatag tgaaattaaa aaaaacagta attttngcca cccattgttg 720 ccngcagaag acaatcanaa gaacctgcag gtaaaacaaa ntctatgcag ggaggtgccc 780 65 518 DNA Homo sapiens Unsure (2)..(2) n = a, c, g or t 65 gnacttncct acatcatttt tatttaaaac acaaacaatt ntttcncttt ctgtangggc 60 aaatggttca aataatgcgg aacacaaaac nttnactaat acaattgctt taaatntnaa 120 acaaaattat tttttaaaaa ancaaaaaaa taaaaaatnt ttncaaangg gacctgaaat 180 ctntaanctn atnccaaaaa caaaataatt naaaaatcca nggtgaaacc tnaacntnct 240 nccnctgctt tggaaaaggg ctntcataca acnttcattc ncctaaaaat ggattggtaa 300 angtttttnt atacataaac tncattcatt tttgcttntg caaaatcanc ccaanctncc 360 caaaactnaa tgggcantcc tntggctttc tncctttccc anatncccaa caaggctact 420 tnaatttcaa cncttnataa nccgcttaca acancatttc cttaggancc aacatnacgg 480 tgggtcaaat cccctataaa aaacaaaact ggccncct 518 66 490 DNA Homo sapiens Unsure (452)..(452) n = a, c, g or t 66 gttaataaag ttggtgagat ccatgtgaag acattagaag aaattcttct tgaaagagcc 60 agtcagaaac gtggagaatt gcaaactaaa ctcaagacag aaggaccttc aaaaactgat 120 gattctactt caggagcaag aagctcctcc actatccgta tcaaaacctt ctctgaggtc 180 ctggctgaaa aaaaacatcg gcagcaggaa gcagagagac aaaaaagcaa aaaggataca 240 acttgcatca agctaaagat tgatagtgaa attaaaaaaa cagtagtttt gccacccatt 300 gttgccagca gaggacaatc agaggagcct gcaggtaaaa caaagtctat gcagggaggt 360 gcacatcaag acgctggaag aaattaaact ggagaaggca ctgagggtgc agcagagctc 420 tgagagcagc accagctccc cgtctcaaca cnaggccact ccaagggcaa ggcggctgct 480 gcnaatcccc 490 67 359 DNA Homo sapiens Unsure (5)..(5) n = a, c, g or t 67 cccanctccn ttttanttna aancccaacc aattnttccc cttccgntan gggcaatngn 60 tccaattatn ncgaacncca aaccttnaan natnccaatt ncttaaatnt taaaccaaat 120 tnntttttta aaaagccaaa naattaagaa ttttttccaa agggaacnng aatccnttag 180 ggtaatccca aaaccaaatt agttaaaaat ccctggntaa acccnaacnt tccnccnccn 240 ccttggaaaa agggnnnccn ncnaccttcc atncncntaa aaatgaatgg ntaaagnttt 300 ttcnnncctt aacntccatc ctttttgnct nttccaaanc ctccccancc tccccaaaa 359 68 611 DNA Homo sapiens Unsure (26)..(26) n = a, c, g or t 68 tgaaaaaaaa catcggcagc aggaancaga aagacnaaaa agcaaaaagg atactacttg 60 catcangcta angattgata gtgaaattaa aaaaacagta tttttgccac ccattgttgc 120 cancagagga caatcanagg agcctgcagg taaaannaag tctatgcagg aggtgcacat 180 caagacgctg gaagaaatta aactggagaa ggcactgagg gtgcagcana gctctgagag 240 cagcaccagc tccccgtctc aacacnaagc cactccatgg gcnangcggc tgctgcgant 300 cnccnaaaga ncagggatga angaagagaa gaaccttcag gaaggaaatg aatttgattc 360 tcagancatt attataactg aagctnnana ngcttcnggt gagaccacng ganttgacat 420 cactaaaatt ccagtcaaga gatgtgagac catgagagag aagcacatgc acaaaacanc 480 nngagaggga aaaatcagtc ttgacacctc ttcggggaga tgtagcatct tgcggnaccc 540 aantggcaga gaaaccagtg ctcactgctg tgccaggaat cacncggcac ctgaccaagc 600 ggcttcccac a 611 69 634 DNA Homo sapiens Unsure (3)..(3) n = a, c, g or t 69 ccnacatcag ttttatttaa aacacaaaca agtntttcnc tttctgtnag ggcaaatggt 60 tcaaataatg cggaacacna aacattgact aatacaantn ctttaaatat gaaacaaaat 120 tattttttaa aaaancnaaa naataaagaa tatntncaaa agggacctgg aatctgtnag 180 ctgattccaa aaacnaaata anttnaaaat ccntggtgaa acctgaacat tctacctctg 240 ctttggaaaa gggntatcat acaacattca gtcngctgaa aatggattgg taaaagtntn 300 tctatacata aacttcagtc atttttgctt gtncaaaatc atcccaatct tcccaaaant 360 gaatgggcag tcctgtggct ttcttccttt tccatattcc caacaaggnt acntnaantt 420 caactcttga nnanccgctt acaacagcag ttccttagga nccccatgac aggtgggtcn 480 aatttcccta tnaaaaacaa aactgggccc tacagcaaaa tatccaaatg ggtnagtcct 540 tccttcctct tcccctgant atatacacat atctcctttc aanaatanta tttccccatg 600 cttattcctt cccnaatcta aaccttgaag tgat 634 70 958 DNA Homo sapiens Unsure (730)..(730) n = a, c, g or t 70 gaaagaaaag catcatgtaa aaatgaaagc caagagatgt gccactcctg taatcatcga 60 tgaaattcta ccctctaaga aaatgaaagt ttctaacaac aaaaagaagc cagaggaaga 120 aggcagtgct catcaagata ctgctgaaaa gaatgcatct tccccagaga aagccaaggg 180 tagacatact gtgccttgta tgccacctgc aaagcagaag tttctaaaaa gtactgagga 240 gcaagagctg gagaagagta tgaaaatgca gcaagaggtg gtggagatgc ggaaaaagaa 300 tgaagaattc aagaaacttg ctctggctgg aatagggcaa cctgtgaaga aatcagtgag 360 ccaggtcacc aaatcagttg acttccactt ccgcacagat gagcgaatca aacaacatcc 420 taagaaccag gaggaatata aggaagtgaa ctttacatct gaactacgaa agcatccttc 480 atctcctgcc cgagtgacta agggatgtac cattgttaag cctttcaacc tgtcccaagg 540 aaagaaaaga acatttgatg aaacagtttc tacatatgtg ccccttgcac agcaagttga 600 agacttccat aaacgaaccc ctaacagata tcatttgagg agcaagaagg atgatattaa 660 cctgttaccc tccaaatctt ctgtgaccaa gatttgcaga gacccacagg actcctgtac 720 tgcaaacaan acaccgtgca cgggctgtga cctgcaaaaa gtacagcaga gctggaggct 780 gaggagctnc gagaaattgc aaccantaca anttccaaag cacgtngaac cttgattccc 840 agaataactt gangggtggg cccaaccttg cccaagaaaa ccaccngtga aancaancca 900 acggagccct antnggcttt gatttgggaa tttgggaaan gaatncaagg gaggngag 958 71 816 DNA Homo sapiens Unsure (510)..(510) n = a, c, g or t 71 gggagggaaa gacaaaacgt atttattcca ggccaggtct taaaatgcac actgcacggt 60 tccctgttgt tatcagcacc agtaaggaaa gaacgtgcct taacggcagc cccacccaga 120 gcctgctgcg tggctgctgt gaggctcccc atgaatccac gcagtcttct tcctcactgg 180 tgcagttggt gaggttttct accctcacag caaagggatc cttaactata aattcacggt 240 atgcagagaa gaggacagaa tctgatttac tgattgttcc tcatttaaac catgacttaa 300 tctctatctt aggatttaac tatctttatt ttctggttaa aatttttaaa aaaagtgggg 360 agagggtgag agtcgtaagg ggcaatagca atagagatta cactgtgctg acacagagac 420 taaattctag tcagagtgaa gaccatataa aaggccggct gatggtttaa aggaagtaac 480 tacatggagt ctaatcgaga cattcatgan ttacatctca ttattagcct tagtaatgta 540 agaaaacaat tctcaacaaa actgggagtc cacagttgtc aagtatgctt tctcangcac 600 gggtaggtaa aagtctggan aaatgggttc tctccatgcc caatgacaaa gcaagacggt 660 cctaggtttg aagttaaaaa caggtcccaa ttgcccgggc ggtatccgcc agctcacagc 720 tgaatttaan catggaaatc caatggaaaa attggganat acnggcacat tcanaaggct 780 ggtcctttga cttatctcca naacccgggt actggc 816 72 726 DNA Homo sapiens Unsure (552)..(552) n = a, c, g or t 72 tgagaaaagt ggtacaaata ctgggaaaaa cctgctcttc tgcgttaagt gggagacaat 60 gtcacaagtt aaaagctctt attcctatga tgccccctcg gatttcatca atttttcatc 120 cttggatgat gaaggagata ctcaaaacat agattcatgg tttgaggaga aggccaattt 180 ggagaataag ttactgggga agaatggaac tggagggctt tttcagggca aaactccttt 240 gagaaaggct aatcttcagc aagctattgt cacacctttg aaaccagttg acaacactta 300 ctacaaagag gcagaaaaag aaaatcttgt ggaacaatcc attccgtcaa atgcttgttc 360 ttccctggaa gttgaggcag ccatatcaag aaaaactcca gcccagcctc agagaagatc 420 tcttaggctt tctgctcaga aggatttgga acagaaagaa aagcatcatg taaaaatgaa 480 agccaagaga tgtgccactc ctgtaatcat cgatgaaatt ctaccctcta agaaaatgaa 540 agtttctaac ancacaaaga agccagagga agaaggcagt gctcatcaag atactgctga 600 aaagaatgca tcttccccaa gagaaagcca agggtagaca tactgtgcct tgtatgccac 660 ctgcanagca gaagtttcna aaangtactg angagcaang aatctggaga agagtatgaa 720 aaatgc 726 73 824 DNA Homo sapiens Unsure (131)..(131) n = a, c, g or t 73 aggatttaac tatctttatt ttctggttaa aatttttaaa aaaagtgggg agagggtgag 60 agtcgtaagg ggcaatagca atagagatta cactgtgctg acacagagac taaattctag 120 tcagagtgaa nacccatata aaaggccggc tgatggttta aaggaagtaa ctacatggag 180 tctaatcgag acattcatga gttacatctc attattagcc ttagtaatgt aagaaaacaa 240 ttctcaacaa aactggagtc cacagttgtc aagtatgctt tctcaggcac gggtaggtaa 300 aagtctggan aaatgggttc tctccatgcc caatgacaaa gcaagacggt cctaggtttg 360 aggttaagan caggtcccat tgccgggcgg tatccgcagc tcacagctga ntttagcagt 420 ggaatcgagt ggagaatttg gggagataca ggcncagtca gaggctggtc acttgacttt 480 atctccagac cctggtactt gcgtattgga tttgccttat gcaccagttc tctccgtagc 540 ctggccanct cctctttttt ctgctcttcc tcctgtagtc tggcctcctc caactgctgg 600 gctttctggg cttctacctc agccattctc ttctccagct ccctgccgct ctttggctct 660 ctctcagtag cccactgaaa angtccctga acnaaaaaaa ccanaaanng gccctcacaa 720 ctgatttcnt ctctttcttg gggaaccaag ggcccctgaa aaaanaaacg gtgtttggaa 780 caaaccntga aacaagcngc ctccttctgc ctgtcccaat tcct 824 74 399 DNA Homo sapiens Unsure (7)..(7) n = a, c, g or t 74 ggtttgnagg gaaaaanaaa actttttttt cccagnccag ttcttaaant nccccnngcn 60 nggtcccctn tntttttcnc ccccattaag gaaaaaactt gcntnancgg nagccccccc 120 caaaacctnc tgcttggctg ctttaaggnc cccataannc cccccatnnt cctcccccac 180 tggtncattg gtnaggtttc ctcccccccn ccaaaggnnt ccttacntat aaatcccngg 240 tttncaaaaa aaaananaaa accaatttcn gatnntcccc cttnaancca gnacttaatc 300 cctntctnag gattnaacaa cctttttttn cgggttaaaa tttttaaaaa aattngggaa 360 anggttaaat ccttaggggg aatnccnata aaaattacc 399 75 826 DNA Homo sapiens Unsure (518)..(518) n = a, c, g or t 75 gtcaaatgct tgttcttccc tggaagttga ggcagccata tcaagaaaaa ctccagccca 60 gcctcagaga agatctctta ggctttctgc tcagaaggat ttggaacaga aagaaaagca 120 tcatgtaaaa atgaaagcca agagatgtgc cactcctgta atcatcgatg aaattctacc 180 ctctaagaaa atgaaagttt ctaacaacaa aaagaagcca gaggaagaag gcagtgctca 240 tcaagatact gctgaaaaga atgcatcttc cccagagaaa gccaagggta gacatactgt 300 gccttgtatg ccacctgcaa agcagaagtt tctaaaaagt actgaggagc aagagctgga 360 gaagagtatg aaaatgcagc aagaggtggt ggagatgcgg aaaaagaatg aagaattcaa 420 gaaacttgct ctggctggaa tagggcaacc tgtgaagaaa tcagtgagcc aggtcaccaa 480 atcagttgac ttccacttcc gcacagatga gcgaatcnaa caacatccta ngaaccagga 540 ggaatataag ggaagtgaac tttacatctg aactacgaaa gcatccttca tctcctgccc 600 gaantgacta aggggatgtt ccattgttaa gcctttcaac ctgtcccngg gaaagaanag 660 aacntttgat gaaacagttt ctacatatgt gccccttgcc cngcaagttg aagacttccn 720 taancgaacc ctnactgatt tcttttgang aaccagaang gntgattttn ccctgtttcc 780 ctccaatctt ctgtgaacaa gatttggccg aanacccccg aacccc 826 76 770 DNA Homo sapiens Unsure (115)..(115) n = a, c, g or t 76 gggaaagaca aaacgtattt attccaggcc aggtcttaaa atgcacactg cacggttccc 60 tgttgttatc agcaccagta aggaaagaac gtgccttaac ggcagcccca cccanagcct 120 gctgcgtggc tgctgtgagg ctccccatga atccacgcag tcttcttcct cactggtgca 180 gttggtgagg ttttctaccc tcacagcaaa gggatcctta actataaatt cacggtatgc 240 anagaanagg acagaatctg atttactgat tgttcctcat ttaaaccatg acttaatctc 300 tatcttagga tttaactatc tttattttct ggttaaaatt tttaaaaaaa gtggggagag 360 ggtgagagtc gtaaggggca atagcaatag agattacact gtgctgacac agagactaaa 420 ttctagtcag agtgaagacc catataaaag gccggctgat ggtttaaagg aagtaactac 480 atggagtcta atcgagacat tcatgagttn catctcatta ttagccttag taatgtaaga 540 aaacnattct caacaaaact ggagtccaca gttgtcaant ntgctttctc aggcacgggt 600 aggtnaaaat ctgganaaat gggttctctc catgcccaat gacaancaan anggtcctag 660 gtttgaagtt aaaaacangt cccattgccg gcggtatccg cagctcacag ctgaatttac 720 cngtggaatc aantggaaaa tttgggaaaa tacnggccca atcaaaaggt 770 77 593 DNA Homo sapiens Unsure (6)..(6) n = a, c, g or t 77 gaaganctga gcagcacagc actggtgaag aagagctgcc tggcggagct cctccggctt 60 tacaccaaaa gcagcagctc tgatgaggag tacatttata tgaacaaagt gaccatcaac 120 aagcaacaga atgcagagtc tcaaggcaaa gcgcctgagg agcagggcct gctacccaat 180 ggggagccca gccagcactc ctcggcccct cagaagagcc ttccagacct cccgccaccc 240 aagatgattc cagaacggaa acagcttgcc atcccaaaga cggagtctcc agagggctac 300 tatgaagagg ctgagccata tgacacatcc ctcaatgagg acggagaggc tgtgagcagc 360 tcctacgagt cctacgatga anaggacggc agcaagggca agtcggcccc ttaccantgg 420 ncctcgccgg aggccggcat cganctgatg cgtgacgccc gcntctgcgc cttcctgtgg 480 cgcaagaaag tggctgggac agtgggccaa gcagctctgt gtcatcnagg acaacaggct 540 tctgtgctnc naatcctcca aggaccccng ccctcagctg gacgtgaacc tac 593 78 1024 DNA Homo sapiens Unsure (668)..(668) n = a, c, g or t 78 aagagattga agcaaatgaa tggaagaaga aatacgaaga gacccggcaa gaagttttgg 60 agatgaggaa aattgtagct gaatatgaaa agactattgc tcaaatgatt gaagatgaac 120 aaaggacaag tatgacctct cagaagagct tccagcaact gaccatggag aaggaacagg 180 ccctggctga ccttaactct gtggaaaggt ccctttctga tctcttcagg agatatgaga 240 acctgaaagg tgttctggaa gggttcaaga agaatgaaga agccttgaag aaatgtgctc 300 aggattactt agccagagtt aaacaagagg agcagcgata ccaggccctg aaaatccacg 360 cagaagagaa actggacaaa gccaatgaag agattgctca ggttcgaaca aaagcaaagg 420 ctgagagtgc agctctccat gctggactcc gcaaagagca gatgaaggtg gagtccctgg 480 aaagggccct gcagcagaag aaccaagaaa ttgaaggaac tgacaaaaat ctgtgatgag 540 ctgattgcaa agctgggaaa gactgactga gacactcccc ctgttagctc aacagatctg 600 catttggctg cttctcttgt gaccacaatt atcttgcctt atccaggaat aattgcccct 660 ttgcaganga aaaaaatata cttaanaaaa gcacatgcct actgctgcct gtcccgcttt 720 gctgccaatg caacagccct ggaagaaaac cctatanggn tgcatagtct aaaaagggag 780 ttgtngactn gacagtgctg ggagcctnct agtttccccc cnatgaaagg ttcccttagg 840 ctgctgagtt tggggtttgt gatttaacct taagtttgtt ttaaagtcca ncttaacttt 900 cccaaattgt gtttaaaatt tgtaacnccc cctttggggt cttcccaaca accggtccga 960 tttttttggn gatcggttta acccttttaa ttttttagta nccagtgggg tttaatttag 1020 ggga 1024 79 734 DNA Homo sapiens Unsure (151)..(151) n = a, c, g or t 79 gagacaagat cttgctgtca cccaggatgg agtgcagtgg catgatcatg gctcactgca 60 gccttgacct cccaggctcc cacctcagcc tcccaagtag ctgggaccac aggcacgtgc 120 caccatgccc agctaatttt tattttggta nanacaaggt ttcaccatgt tgcctaggta 180 ggtttcaaac tcctggactc aagtgatcct cctgcctcgg ccttccacag tgttgggatt 240 acaggaataa gccactgtgc ccggcccttt ttctcttctg taacagantt tattactgcc 300 tagctagcag gttatttggc cctcacatgt gttgaggcaa actctatact atattcttac 360 tctccanagt tccaaaatcc tttattttta aanaaaaata aacaaacata cttcattctg 420 cccagtatat tctcttgatc tgtacaagct acgattttaa ttctctttgg gagaggaagc 480 atctgttaag ttcgaatggg ggatatttcc tcataacggt catggctgan aagccaggac 540 aattatcact taacgaaggt cctttggtgc tccctgtgca tcagcttcat tcactggggt 600 caggttctta aggggtctct tccaccaatg tgctagggaa gggctgccat cacctctgtt 660 taacacatag ctactttctt aaaccnataa gcttaaaaaa gangactatg gaattaccaa 720 tggaaggcnt ataa 734 80 592 DNA Homo sapiens Unsure (9)..(9) n = a, c, g or t 80 gttatgaana cctttccaaa ttcatttgta tttctgttaa atttattttt tacttttaga 60 gtggctatca ttataatgta atttaaaatt atatttgtaa aagtgactat tggagtgagt 120 acgaattttg tttatanatc tatgataaat gcattctccc tntaggaggt agaanagtat 180 acagctgtnt ataataagct tcgctatgaa catacatttc tcaagtcaga atttgaacac 240 cagaaggaag agtatgcacg tnttttagat gaangaaacn ataaactatg aatcagagat 300 ngcaanactg gaggaagatn aagaagaact acgtanccag ctgcttaatg tngatctcac 360 anaagacagc aaacgagtgg aacaacttgc tcgagaaaaa gtctatttgt gtccaaaatt 420 aagangttta gaggctgaag taccngaatt aaaggctgaa naggagaatt ctgangctca 480 ggtggaaaat gcccaaanaa tacacgtgcg gcagttggct gagatgcacg ctacagtcag 540 atccctggag gctgacaanc aatcanctaa tttacgggca naacgcttgg aa 592 81 373 DNA Homo sapiens Unsure (66)..(66) n = a, c, g or t 81 aaaaaaaaat taagctcttt aattatgtgc acacagattt tagaaaaggt agccttttgt 60 atatanatac ctttacattc tttaggntga nttttaaatt gtcatctttt ttcaactaca 120 gtttttgtnt atagtaaacc anaanatgtg tntggaccct gttatggnca agcatctcaa 180 agatgaagan agaattaatg atagttatat ttcactcaaa atgccaaaaa aaaaaattca 240 acaaagtaaa aattttaaaa cttgactcta actagttcct ttttgtttta cattctcaaa 300 ccattgtnaa atattctaaa tatctctgaa aatttctctt ttaatgcttc acttgtntaa 360 tcttaaaatc ctg 373 82 661 DNA Homo sapiens Unsure (551)..(551) n = a, c, g or t 82 cacacctggt ggtcctgaag acagcccagg acccagggat ctcccccagc cagagtctgt 60 gtgcggaaag ttccagaggc ctcagtgcag gctccctgtc ggagagtgca gttgggcccg 120 tggaggcatg ctgcctggtc atcctggctg cagagagcaa ggtcgctgcg gaggagcttt 180 gctgtctgct aggccaggtc ttccaggttg tttacacgga gtccaccatc gactttctgg 240 acagagcgat atttgatggg gcctctaccc cgacccacca cctgtccctg cacagcgatg 300 actcttctac aaaagtggac attaaggaga cctacgaggt ggaagccagc actttctgct 360 tccctgaatc tgtggatgtg ggtggtgcat caccccacag caagaccatc agtgagagcg 420 agctgagcgc cagcgccact gagctgctgc aggactacat gctgacgctg cgcaccaagc 480 tgtcatcaca ggagatccag cagtttgcag cactgctgca cgagtaccgc aatggggcct 540 ctatccacga nttctgcatc aacctgcggc agctctacgg ggacagccgc aagttcctgc 600 tgcttggtct gaagcccttc atccctgaaa angacagcca gcacttcnag aacttcctgg 660 a 661 83 401 DNA Homo sapiens Unsure (189)..(189) n = a, c, g or t 83 aaattattca cattgcagta aacttctttt taaggtctct gaaagttaca ataggaacat 60 catgtgcaaa actgacagcc gtccaagggc ccagccgaca ggactggctc tccctgcccg 120 ctcggccggg ccctccccga gcggggacac actgcagggc ttggctgaac cctggtggac 180 aaggcaaana nccttccacc ccgcactgag gctcgtgtcc ctcggcagct ccctgctcct 240 tcacagtaaa ngacctgggc cgcccggggc catctgcacc gggcgcctct ccctggccac 300 caccaagggc tgacacgcag gtctgggcag ctccttctgg gaaggcctat gacgactgcg 360 ccgaaggtgt gggtgccccc ccatccactg tccatcatgc c 401 84 1347 DNA Homo sapiens Unsure (460)..(460) n = a, c, g or t 84 aagatcagcg atatcacgcg tcccccggag catcgcgtgc aggagccatg gcgcgggagc 60 tataccacga agagttcgcc cgggcgggca agcaggcggg gctgcaggtc tggaggattg 120 agaagctgga gctggtgccc gtgccccaga gcgctcacgg cgacttctac gtcggggatg 180 cctacctggt gctgcacacg gccaagacga gccgaggctt cacctaccac ctgcacttct 240 ggctcggaaa ggagtgttcc caggatgaaa gcacagctgc tgccatcttc actgttcaga 300 tggatgacta tttgggtggc aagccagtgc agaatagaga acttcaagga tatgagtcta 360 atgactttgt tagctatttc aaaggcggtc tgaaatacaa ggctggaggc gtggcatctg 420 gattaaatca tgttcttacg aacgacctga cagccaagan gctcctacat gtgaagggtc 480 gtanagtggt gagagccaca gaattcccct tagctgggac agtttcaaca agggtgactg 540 cttcatcatt gaccttggca ccgaaattta tcanttggtg tggttcctcn tgcaacaaat 600 atgaacgtct gaaggcaaac caggtagcta ctggcattcg gtacaatgaa aggaaaggaa 660 ggtctgaact aattgtcgtg gaanaaggaa gtgaaccctc agaacttata aaggtcttag 720 gggaaaagcc agagcttcca gatggaggtg atgatgatga cattatanca gacataagta 780 acaggaaaat ggctaaacta tacatggttt cagatgcaag tggctccatg agagtgactg 840 tggtggcana agaaaacccc ttctcantgg caatgctgct gtctgaagaa tgctttattt 900 tggaccacgg ggctgccaaa caaattttcg tatggaaagg taaagatgct aatccccaag 960 agaggaaggc tgcaatgaag acagctgaag aatttctaca gcaaatgaat tattccaaga 1020 atacccaaat tcaagttctt ccagaaggag gtgaaacacc aatcttcaaa cagtttttta 1080 aggactggag agatnaacga tcagagtgat ggcttcggga aagtttatgt cacagagaaa 1140 gtggctcaan tnnaacnaat tccctttgat gcctcnnaat tacncagttc tccgcagatg 1200 gcagcccagc acaatatggt ggatgatggt tctggccaag tggaaatttg gcgtgtncaa 1260 aacaatggta ggatccaagt tgaccnnaac tcctatggtg actcccatgg tggtgactgc 1320 tacttcatac tctacaccta tccctga 1347 85 3441 DNA Homo sapiens Unsure (2959)..(2959) n = a, c, g or t 85 atgtttctaa cattgaactc taaggaagct ggtgaacaaa cacgccatat gtatgcagaa 60 cacttaacag aattatgcta tgttgtctgt ttttgtttgt atttcttgtc cttgctgaag 120 attgacttga aatcttaaac taagttctcc ctctttatag gcggtgacag tgatcctcca 180 ttaaagcgta gcctggcaca gaggctaggg aagaaagttg aagctccaga aactaacatt 240 gacaaaacac caagaaaagc tcaagtttcc aagtctctta agggagcgat taggcatgtc 300 agctgatcca gataatgagg atgcaacaga taaagttaat aaagttggtg agatccatgt 360 gaagacatta gaagaaattc ttcttgaaag agccagtcag aaacgtggag aattgcaaac 420 taaactcaag acagaaggac cttcaaaaac tgatgattct acttcaggag caagaagctc 480 ctccactatc cgtatcaaaa ccttctctga ggtcctggct gaaaaaaaac atcggcagca 540 ggaagcagag agacaaaaaa gcaaaaagga tacaacttgc atcaagctaa agattgatag 600 tgaaattaaa aaaacagtag ttttgccacc cattgttgcc agcagaggac aatcagagga 660 gcctgcaggt aaaacaaagt ctatgcaggg aggtgcacat caagacgctg gaagaaatta 720 aactggagaa ggcactgagg gtgcagcaga gctctgagag cagcaccagc tccccgtctc 780 aacacgaggc cactccaagg gcaaggcggc tgctgcgaat ccccaaaaga acagggatga 840 aagaagagaa gaaccttcag gaaggaaatg aatttgattc tcagagcatt attataactg 900 aagctaaaga agcttcaggt gagaccacag gagttgacat cactaaaatt ccagtcaaga 960 gatgtgagac catgagagag aagcacatgc acaaaacaac aggagaggga aaaatcagtc 1020 ttgacacctc ttcggggaga tgtagcctct tgcaataccc aagtggcaga gaaaccagtg 1080 ctcactgctg tgccaggaat cacacggcac ctgaccaagc ggcttcccac aaagtcatcc 1140 cagaaggtgg aggtagaaac ctcagggatt ggagactcat tattgaatgt gaaatgtgca 1200 gcacagacct tggaaaaaag gggtaaagct aaacccaaag tgaacgtgaa gccatctgtg 1260 gttaaagttg tgtcatcccc caaattggcc ccaaaacgta aggcagtgga gatgcacgct 1320 gctgtcattg ccgctgtgaa ccactcagct ccagcagtgt cctacaggaa cccccagcca 1380 aaaaggcagc tgtggctgtt gtcccgcttg tctctgagga caaatcagtc actgtgcctg 1440 aagcagaaaa tcctagagac agtcttgtgc tgcctccaac ccagtcctct tcagattcct 1500 cacccccgga ggtgtctggc ccttcctcat cccaaatgag catgaaaact cgccgactca 1560 gctctgcctc aacaggaaag cccccactct ctgtggagga tgattttgag aaactaatat 1620 gggagatttc aggaggcaaa ttggaagctg agattgacct ggatcctggg aaagatgaag 1680 atgaccttct gcttgagcta tcagaaatga ttgatagctg aagggtggta gtgaggacac 1740 tttaaaaaaa aatcgccaaa aaactggact tagtttcatc tattgtaaca tttacctgag 1800 atgatcattt ctttagtcta gaatttgccc caaatcagaa gtatacctct gaattatctg 1860 tatgtgtcct ggattccttg gggtcagatt tttaaagtta ctttataacc attttgtcca 1920 tttgatgcca ttgtttatca tcttttgaga aaaaagttct gtcataccct tctctccaca 1980 aaaaagagac tgagagggag atcaagtgaa agggtgcaag cgaacttagt gactccttga 2040 ggtgtttgtc agttttggct tttttcttct ttgttgtatt ctttatgtat tgtcttgatg 2100 tacttaatat tacctgagtt tgaaatggat gaagacagct gctaccatta aggaccaaat 2160 tttatgctac cactaaacaa aaatacccac tcagtctgtg ttaaattgta tgtcttttta 2220 aaggtattta aagattcaac taagctttaa agagggctga gcagctcagg aagcctgtaa 2280 tgtgggcata actctttgga cctgatcttg atgcttctgc tgctctgtta gcctctgaag 2340 agcaatatct aatttattat tactgtaatt ttttaaaagg ctttaaagtg cctcaggggt 2400 cccctgaaac taattttcta tttctgggat tccctggatt cattatatga gatggtgaca 2460 tgattagagg aattcttttt tagtatgaaa attgtccctt ttcttcttca gtacttgcct 2520 ccttgctggc attgaattaa cacagggaca aaatttggtt aattttttat ttctaactct 2580 cccaacaaac ccctgttgcc cagtatttgt ttggtggcct ttaaccacct gagggaaaaa 2640 atgagcttat tcaagctgcc aatatttatc tatgggctgt agcagtacac tgaattgtac 2700 tgtgccaggg atattgagat gctctggggg tgtattgtat acctgccagt tttcttcatt 2760 tctgaattga gttttctttt cttgatgttg gtttccttca tatcacctca aggtttagat 2820 ttgtgaagga ataagcatga tggaaataat agtcttgaaa ggagatatgt tgtatataat 2880 caggaggaag aggaaggaag gacttaccca ttttgatatt ttgctgtagg tggccagttt 2940 tgtttctcat agggaaatnt gacccacctg tcatgttggc tccctaagga actgctgttg 3000 taagcggctc atcaagagtt gaacttcacg tagccttgtt gggaatatgg aaaaggaaga 3060 aagccacagg actgcccatt cagttttggg aagattggga tgattttgca caagcaaaaa 3120 tgactgaagt ttatgtatag acacaccttt accaatccat nttcagctga ctgaatgttg 3180 tatgatagcc cttctccaaa gcagaggtag aatgttcagg tttcaccatg gattttctac 3240 ttatttcgtt tttggaatca ccttacagat tccaggtccc ttttgtatat attctttatt 3300 cttttgcttt tttaaaaaat aattttgttt catatttaaa gcacttgtat tagtcaatgt 3360 ttcgtgttcc gcattatttg aaccatttgc ccttacagaa agagaaatac ttgtttgtgt 3420 tttaaataaa actgatgtag g 3441 86 1016 DNA Homo sapiens Unsure (691)..(691) n = a, c, g or t 86 gtcaaatgct tgttcttccc tggaagttga ggcagccata tcaagaaaaa ctccagccca 60 gcctcagaga agatctctta ggctttctgc tcagaaggat ttggaacaga aagaaaagca 120 tcatgtaaaa atgaaagcca agagatgtgc cactcctgta atcatcgatg aaattctacc 180 ctctaagaaa atgaaagttt ctaacaacaa aaagaagcca gaggaagaag gcagtgctca 240 tcaagatact gctgaaaaga atgcatcttc cccagagaaa gccaagggta gacatactgt 300 gccttgtatg ccacctgcaa agcagaagtt tctaaaaagt actgaggagc aagagctgga 360 gaagagtatg aaaatgcagc aagaggtggt ggagatgcgg aaaaagaatg aagaattcaa 420 gaaacttgct ctggctggaa tagggcaacc tgtgaagaaa tcagtgagcc aggtcaccaa 480 atcagttgac ttccacttcc gcacagatga gcgaatcaaa caacatccta agaaccagga 540 ggaatataag gaagtgaact ttacatctga actacgaaag catccttcat ctcctgcccg 600 agtgactaag ggatgtacca ttgttaagcc tttcaacctg tcccaaggaa agaaaagaac 660 atttgatgaa acagtttcta catatgtgcc nccttgcaca gcaagttgaa gacttccata 720 aacgaacccc taacagatat catttgagga gcaagaagga tgatattaac ctgttaccct 780 ccaaatcttc tgtgaccaag atttgcagag acccacagac tcctgtactg caaaccaaac 840 accgtgcacg ggctgtgacc tgcaaaagtt acagcagagc tggaggctga ggagctcgag 900 aaattgcaac aatacaaatt caaagcacgt gaacttgatc ccagaatact tgaaggtggg 960 cccatcttgc ccaagaaacc acctgtgaaa ccacgccgag ccctatgcct cgtgcc 1016 87 1135 DNA Homo sapiens Unsure (27)..(27) n = a, c, g or t 87 aggaattggg acaggcagaa ggaggcngct tgtttcangg tttgttccaa acaccgtttn 60 ttttttcagg ggcccttggt tccccaagaa agagangaaa tcagttgtga gggccnnttt 120 ntggtttttt tngttcaggg acnttttcag tgggctactg agagagagcc aaagagcggc 180 agggagctgg agaagagaat ggctgaggta gaagcccaga aagcccagca gttggaggag 240 gccagactac aggaggaaga gcagaaaaaa gaggagntgg ccaggctacg gagagaactg 300 gtgcataagg caaatccaat acgcaagtac cagggtctgg agataaagtc aagtgaccag 360 cctctgactg ngcctgtatc tccccaaatt ctccactcga ttccactgct taaattcagc 420 tgtgagctgc ggataccgcc cggcaatggg acctgttttt aacttcaaac ctaggaccgt 480 cttgctttgt cattgggcat ggagagaacc catttntcca gacttttacc tacccgtgcc 540 tgagaaagca tacttgacaa ctgtggactc cagttttgtt gagaattgtt ttcttacatt 600 actaaggcta ataatgagat gtaactcatg aatgtctcga ttagactcca tgtagttact 660 tcctttaaac catcagccgg ccttttatat gggtcttcac tctgactaga atttagtctc 720 tgtgtcagca cagtgtaatc tctattgcta ttgcccctta cgactctcac cctctcccca 780 ctttttttaa aaattttaac cagaaaataa agatagttaa atcctaagat agagattaag 840 tcatggttta aatgaggaac aatcagtaaa tcagattctg tcctcttctc tgcataccgt 900 gaatttatag ttaaggatcc ctttgctgtg agggtagaaa acctcaccaa ctgcaccagt 960 gaggaagaag actgcgtgga ttcatgggga gcctcacagc agccacgcag caggctctgg 1020 gtggggctgc cgttaaggca cgttctttcc ttactggtgc tgataacaac agggaaccgt 1080 gcagtgtgca ttttaagacc tggcctggaa taaatacgtt ttgtctttcc ctccc 1135 

1. A method for determining gastric cancer or a related condition in a sample, comprising assaying said sample for a sterol carrier protein-X/sterol carrier protein-2 protein or a Protein Kinase B/AKT protein, presence of said protein in said sample being indicative of the gastric cancer or the condition.
 2. The method of claim 1, wherein the sterol carrier protein-X/sterol carrier protein-2 protein is encoded by a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 19, 20, 21, and
 22. 3. The method of claim 1, comprising assaying said sample for a peptide derived from said sterol carrier protein-X/sterol carrier protein-2 protein.
 4. The method of claim 1, comprising assaying said sample for an antigenic fragment of said sterol carrier protein-X/sterol carrier protein-2 protein.
 5. The method of claim 1, wherein the protein is assayed using an antibody that specifically binds sterol carrier protein-X/sterol carrier protein-2.
 6. The method of claim 1, wherein the Protein Kinase B/AKT protein is encoded by a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:13 and
 14. 7. The method of claim 1, comprising assaying said sample for a peptide derived from said Protein Kinase B/AKT protein.
 8. The method of claim 1, comprising assaying said sample for an antigenic fragment of said Protein Kinase B/AKT protein.
 9. The method of claim 1, wherein the protein is assayed using an antibody that specifically binds Protein Kinase B/AKT.
 10. A method for determining gastric cancer or a related condition in a sample, comprising assaying said sample for expression of a nucleic acid molecule which encodes sterol carrier protein-X/sterol carrier protein-2 or Protein Kinase B/AKT, as a determination of the gastric cancer or therelated condition in said sample.
 11. The method of claim 10, wherein the nucleic acid molecule that encodes sterol carrier protein-X/sterol carrier protein-2 comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs:19, 20, 21, and
 22. 12. The method of claim 10, wherein the nucleic acid molecule that encodes Protein Kinase B/AKT comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs:13 and
 14. 13. A method for determining gastric cancer or a related condition in a sample, comprising assaying said sample for an antibody that specifically binds sterol carrier protein-X/sterol carrier protein-2 or Protein Kinase B/AKT, as a determination of the condition in said sample.
 14. The method of claim 13, wherein the sterol carrier protein-X/sterol carrier protein-2 is encoded by a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 19, 20, 21, and
 22. 15. The method of claim 13, wherein the antibody is assayed using sterol carrier protein-X/sterol carrier protein-2 or an antigenic fragment thereof.
 16. The method of claim 13, wherein the Protein Kinase B/AKT is encoded by a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:13 and
 14. 17. The method of claim 13, wherein the antibody is assayed using Protein Kinase B/AKT or an antigenic fragment thereof.
 18. A method for determining regression, progression or onset of a gastric cancer or a related condition, comprising monitoring a sample from a patient with said gastric cancer or related condition for a parameter selected from the group consisting of (i) a sterol carrier protein-X/sterol carrier protein-2 or Protein Kinase B/AKT protein, (ii) a peptide derived from said protein and (iii) cytolytic T cells specific for a peptide derived from said protein and an MHC molecule to which the peptide is bound, which is indicative of progression or regression or onset of said gastric cancer or a related condition.
 19. The method of claim 18, wherein said sample is a body fluid or effusion.
 20. The method of claim 18, wherein said sample is a tissue.
 21. The method of claim 18, wherein monitoring comprises contacting said sample with an antibody that specifically binds with said protein or peptide.
 22. The method of claim 21, wherein said antibody is labeled with a radioactive label or an enzyme.
 23. The method of claim 21, wherein said antibody is a monoclonal antibody.
 24. The method of claim 18, comprising amplifying RNA which codes for said protein.
 25. The method of claim 24, wherein said amplifying comprises carrying out polymerase chain reaction.
 26. The method of claim 18, comprising assaying said sample for said peptide.
 27. The method of claim 18, comprising contacting said sample with a nucleic acid molecule which specifically hybridizes to a nucleic acid molecule which codes for or expresses said sterol carrier protein-X/sterol carrier protein-2 protein.
 28. The method of claim 27, wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs:19, 20, 21, and
 22. 29. The method of claim 18, comprising contacting said sample with a nucleic acid molecule which specifically hybridizes to a nucleic acid molecule which codes for or expresses said Protein Kinase B/AKT protein.
 30. The method of claim 29, wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs:13 and
 14. 31. A method for following progress of a therapeutic regime designed to alleviate gastric cancer or a related condition, comprising: (a) assaying a sample from a subject to determine level of a parameter selected from the group consisting of (i) a peptide derived from a sterol carrier protein-X/sterol carrier protein-2 or a Protein Kinase B/AKT protein, (ii) a cytolytic T cell specific for cells presenting said peptide, and (iii) an antibody which specifically binds to said peptide of said protein, at a first time period; (b) assaying level of the parameter selected in (a) at a second period of time and comprising it to the level determined in (a) as a determination of effect of said therapeutic regime.
 32. The method of claim 31, wherein the condition is gastric cancer. 